Posted by admin on Feb 29, 2020 in |
The objective of the present study is to develop, evaluate and compare potent phytoconstituents of curcumin loaded solid lipid nanoparticles (CUR-SLN) and Paclitaxel loaded solid lipid nanoparticles (PTX-SLN) for the treatment of malignancy. Curcumin is an active phenolic compound of Curcuma longa as a promising phytoconstituent has been extensively studied over the past several years. The result of these studies has indicated that the curcumin (CUR) acts on multiple molecular targets to selectively kill tumor cells with low intrinsic toxicity. The well-known anticancer agent Paclitaxel (PTX) is used for its effectiveness in the treatment of a wide range of tumors cells. However, due to the hydrophobic nature of PTX limits its effective application as anticancer drug. This study utilizes the immense potential of nanotechnology, particularly SLNs, in improving safety & bioavailability of CUR and PTX. SLN was chosen due to their excellent lipoidal drug carrier properties, physiological compatibility, and economic standpoint. In this study physical mixture of CUR-SLN and PTX-SLN formulation and PTX-SLN formulation were examined on Ehrlich ascites...
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Posted by admin on Feb 29, 2020 in |
Rice (Oryza sativa L.) is one of the most important cereal crops consumed in Asia and the primary staple food being consumed by nearly half of the world’s population. Black rice is getting popular recently and is consumed as a functional food. Black rice contains relatively high anthocyanin in the pericarp layer which gives the dark purple color. Black rice has a higher content of phenolic compounds as compared to white rice. The total phenolic content was four times higher in pigmented rice than in non-pigmented varieties. Heat Moisture Treatment (HMT) and enzymatic modification are essential processes prior to absorption in the human body as they affect digestibility. On the basis of these considerations, the dual modification applied in this study was a combination of heat-moisture treatment (HMT) and enzymatic modification. The samples used for the study were native and dual modified rice flour of selected white and black rice variety. The samples were termed as Native White Rice Flour (NWRF), Dual Modified White Rice Flour (DMWRF), Native Black...
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Posted by admin on Feb 29, 2020 in |
Arsenic and mercury are presently the most common pollutants of freshwater bodies. There is a continuous increase in the toxic level of these pollutants with some seasonal changes, affecting the aquatic biota. The present study aimed to identify cellular biomarkers of arsenic and mercury toxicity in freshwater fish Channa punctatus. Antioxidant defense like catalase (CAT), superoxide dismutase (SOD), ascorbate, reduced glutathione (GSH), oxidative stress marker lipid peroxidation (LPO), lysosomal marker like acid phosphatases and apoptotic marker namely caspases-3 were employed to check the damage caused to the fish as a result of arsenic and mercury contamination of water. Results indicate that increased lipid peroxidation induced apoptosis in arsenic toxicity. On the other hand, mercury toxicity induced necrosis mediated by lysosomal induction. These results further significantly indicate the protective effects of ascorbic acid that reduces the increased level of hepatic oxidative stress during metal toxicity. This study suggests that ascorbic acid supplementation can be a good option to save fish, which are at high risk of heavy metal-induced damage resulting...
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Posted by admin on Feb 29, 2020 in |
Radioisotopic imaging based on small molecules like carbohydrate has been regarded as a promising candidate for in-vivo imaging for cancer diagnosis in recent years. In this study, we report that N-acetyl neuraminic acid (Neu5Ac), a type of sialic acid with 9-carbon amino sugar and low pKa value coordinates with the transition radioactive metal 99mTc. The radiochemical yield of 99mTc labeled Neu5Ac was observed to be greater than 90% and was confirmed by Instant Thin Layer Chromatography. Molecular docking studies showed greater affinity of 99mTc labeled Neu5Ac towards the lectin receptor when compared to cold Neu5Ac. The radiolabeled complex (99mTc-Neu5Ac) binds specifically to the HT-29 cells and was mildly cytotoxic at a concentration of 402 µM. Cellular internalization of 99mTc-Neu5Ac was mainly in the cytosolic proteins and free membranes as compared to nuclear fraction and large organelles. In conclusion, Neu5Ac was successfully radiolabeled with 99mTc, and in-vitro binding studies confirmed that our developed radionuclide probe binds selectively to the cancer cells. Further, efficacy of our developed complex may be useful...
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Posted by admin on Feb 29, 2020 in |
To achieve initial conformation of the observed in-vivo antiepileptic activity by utilization of molecular target gamma-aminobutyric acid (GABA) with the help of software i.e., Molegro Virtual Docker ver. 6.0 molecular docking studies were performed. According to the docking studies it was found that the title compounds of series combine and bind precisely with 1OHY subunits of gamma-aminobutyric acid (GABA), which is accountable for antiepileptic activity. On the basis of the study, it was found that Compound C1, C3, C5, C11, C16, C17, C19, C23, and C24 showed good binding interaction. The results were produced on the basis of evaluation of three scoring functions i.e. mol dock score (-115.737, -102.781, -94.5148, -94.9588, -96.8756, -93.8359, -74.5031, -99.2451 and -89.0893), re-rank score (-72.9177, -81.2145, -59.4376, -81.2145, -59.1531, -80.1486, -63.2278, -61.2916 and -75.9803) and hydrogen bonding (-6.67928, -10.0939, -7.40405,-8.47986, -12.952, -8.39746, -5.77098, -13.9623 and -8.37248) which was very close to reference drug phenytoin which having Moldock score, re-rank score and hydrogen bonding score -76.1363, -60.0613 and -6.83431...
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