Posted by admin on Dec 31, 2019 in |
Protocatechuic acid (PCA) is a type of widely distributed naturally occurring phenolic acid, commonly found in bran, grain, brown rice, fruits such as plums, gooseberries, grapes and also in onion peels. A new, simple, precise, accurate and sensitive stability-indicating HPTLC method for Protocatechuic acid was successfully developed. This method is based on HPTLC separation followed by UV detection at 258 nm. The HPTLC method is used to determine the presence and quantify the protocatechuic acid in onion peel extract. The separation was carried out on Merck TLC aluminum sheets precoated with silica gel 60F254 using Toluene: Ethyl Acetate: Formic acid (6:6:1.2 v/v/v) as a mobile phase and scanning was done by using TLC Scanner III. Protocatechuic acid gave well defined and sharp peak at Rf 0.52 ± 0.03 at 258 nm. The calibration curve was linear in range 100-500 ng/band. Protocatechuic acid was subjected to stress conditions like hydrolysis under acidic, basic and neutral conditions, oxidation, heat, and...
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Posted by admin on Dec 31, 2019 in |
Ranitidine hydrochloride (RHCl) is a histamine H2 receptor antagonist, it’s widely in active duodenum ulcers, gastric ulcers, Zollinger-Ellison syndrome, gastroesophageal reflux disease, and erosive esophagitis. Ranitidine HCl has a short biological half-life of drug 2 – 3 h, has 50% absolute bioavailability, colonic metabolism of ranitidine HCl was partly responsible for the poor bioavailability. Based on these, gastroretentive drug delivery system (GRDDS) using floating-mucoadhesive system dosage form of Ranitidine HCl has been developed that makes less frequent administering of the drug also improve bioavailability. Factorial design 23 was applied to optimize the formula of ranitidine HCl floating-mucoadhesive tablet by the varying level of polymer, it was chitosan 50 – 100 mg as a mucoadhesive agent, HPMC K4M 20 – 50 mg as a swelling agent, and drug release controlled, and sodium bicarbonate 20 – 40 mg as gas generating agent. The optimum formula determined by superimposed contour plot from various parameters: physical properties of granule, tablet, and drug release 6 h using a design expert ® program. Based on...
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Posted by admin on Dec 31, 2019 in |
The objective of the present investigation was the bioprospection of pharmaceutically vital bioactive compounds from endophytic Thielaviopsis basicola with antagonistic and antioxidant activity isolated from Ximenia americana, Western Ghats of Karnataka, India. The fresh and healthy leaves and roots of Ximenia americana were collected from the forests of Western Ghats of Karnataka, India and submerged for the enumeration and Purification of fungal endophytes by using different media. Isolated fungal endophytes were processed for the bioprospection of pharmaceutically vital bioactive compounds by referring to standard protocols. At a glance, endophyte derived bioactive compounds were used in-vitro antagonistic and antioxidant activity. A total of 32 pure isolates were obtained, 8 morphologically distinct fungal endophytes were identified from 200 explants of X. americana. Endophytic Thielaviopsis basicola was a core candidate identified and cultivated in submerged fermentation in potato dextrose broth under the controlled condition at 25 ℃ ± 2℃ for 15 days in incubator shaker. From this fermented medium, 3.30 g of aqueous crude extract per 100 ml of potato dextrose broth...
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Posted by admin on Dec 31, 2019 in |
Green synthesis is one of the best routes for the silver nanoparticles (AgNPs). The present study revealed that the aqueous leaf extracts of Aeschynomene aspera, which contains alkaloids, flavonoids, phenols, terpenoids, anthocyanidins, indoles, glycosides, saponins and tannins, is found to be responsible for bioreduction during the synthesis of silver nanoparticles (SNPs). The colour change from light yellow to dark brown within 20 to 60 min indicates the formation of nanoparticles. The UV-Visible spectrum of the aqueous medium containing silver nanoparticles showed a Surface Plasmon Resonance peak at 450 nm. FT-IR analysis reveals that the phenols and amides are mainly responsible for reduction. XRD analysis clearly showed the characteristic Bragg peaks of (111), (200), (220), (311) facets of the face-entered cubic structure of silver nanoparticles and confirmed that these nanoparticles are crystalline in nature.HR-TEM analysis revealed that the green synthesized SNPs were spherical in shape with an average size of 10 nm. The antibacterial effect of synthesized AgNPs on different bacterial strains with the zone of inhibition 14.1 mm on...
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Posted by admin on Dec 31, 2019 in |
Organophosphorus (OP) nerve agents blocks acetylcholinesterase and do not allow the degradation of acetylcholine. Post-treatment of organophosphate poisoning involves the treatment with oxime reactivators as an antidote, which will dephosphorylate the phosphate bond between enzyme and nerve agent. Due to CNS permeability failure of these oximes, the introduction of low molecular non-oximes has become a challenging role for reactivation of inhibited acetylcholinesterase. In-silico high-throughput structure-based virtual screening and docking by using LOPAC database targeting CNS for inhibited acetylcholinesterase reactivation. For the identification of an active site of AChE enzyme and high throughput virtual screening, Inventus™ software was being used. ADME analysis for the selection of leads which can follow up Lipinski rule of 5 and CNS parameters for permeation. Further docking studies of selected lead candidates was done using Schrodinger software. By taking various CNS parameters into consideration hits were selected. For further refinement docking was performed, and the best lead obtained with glide XP docking score -11.656 Kcal/mol. The best lead candidates passed CNS permeation parameters range and...
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