Posted by admin on Dec 31, 2019 in |
PTEN (phosphatase and tension homolog deleted on chromosome 10) is an important key factor in regulating intracellular for cell growth and proliferation by blocking the phosphatidylinositol -3kinase/ AKT protein kinase B (PI3K/Akt) pathway alteration in PTEN is concomitant with many diseases. PTEN is recurrently mutated in several pathological diseases which indicate its significance in general physiological activity. Various cellular proteins are initiated by phosphorylation; in contrast, PTEN is deactivated based on phosphorylation by specific kinases. Therefore, the development of therapeutic agents includes selectively targeting quickness and kinase-domain containing proteins affecting PTEN associated diseases. The present review focuses on the role of PTEN in pathological conditions and a comprehensive list of currently identified modulators of PTEN and explains potential new molecular targets which could be help in the development of drugs for the management of PTEN associated diseases like cardiovascular diseases, diabetes, obesity, cancer, autism, and Alzheimer’s...
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Posted by admin on Dec 31, 2019 in |
The aim of this research was to perform phytochemical screening and evaluation of the pharmacological activities of Buchanania lanzan Spreng. leaves. Preliminary phytochemical screening revealed the presence of phytoconstituents like steroids, tannins, saponins, carbohydrates, alkaloids, and flavonoids. Three leaf extracts were prepared by using solvents e.g. chloroform, ethyl acetate, and ethanol. The antibacterial activity was evaluated by using the paper disc method against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The chloroform extract was found to be more effective against Pseudomonas aeruginosa with a zone of inhibition 20 mm as compared to the standard anti-biotic ampicillin with a zone of inhibition 20 mm. Antifungal activities were evaluated by well diffusion assay against Candida albicans, Aspergillus flavus, Aspergillus niger. The chloroform extract was found be more effective against Aspergillus flavus and Candida albicans with a zone of inhibition 22 mm and 20 mm respectively as compared to the standard antifungal ketoconazole with a zone of inhibition 22 mm. Antifungal activity against Aspergillus niger with zone of inhibition 22 mm was...
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Posted by admin on Dec 31, 2019 in |
The research herbal formulation prepared by mixing dried stem barks of Azadirachta indica A. Juss. and Saraca asoca Roxb. in equal amounts is standardized through pharmacognostic and phytochemical studies for assessing its efficacy in the treatment of leucorrhoea/vaginitis/ excessive white discharge. Pharmacognostical analysis revealed total ash value of 9.43% having 1.89% acid insoluble ash, 4.62 pH and 7.70% moisture content and presence of nitrogen and halogens. Flavonoids, tannins, carbohydrates, glycosides, saponins and fixed oils were found present in the hydro-alcoholic extracts. The results also showed a high concentration of flavonoids compounds (43.37 µg quercetin equivalent/mg) and high phenolic content (101.22 µg gallic acid equivalent/mg). UV-Visible spectroscopy scanning showed a prominent peak at 277 nm in the hydro-alcoholic extract. HPTLC analysis at 254 nm, 277 nm, and 330 nm indicated the presence of tannic acid, gallic acid, quercetin and catechol. Similarly, HPLC analysis at 277 nm suggested the presence of gallic acid, tannic acid and catechol. FTIR analysis exhibited the presence of N-H stretching, C-H stretching, N-H bending, N-O asymmetric...
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Posted by admin on Dec 31, 2019 in |
Analytical method for simultaneous estimation of Ertugliflozin (ERT) and Metformin hydrochloride (MET) was developed and validated by high-performance liquid chromatography (HPLC) as per ICH guidelines. The drugs were injected into the inertsil C18 (250 × 4.6 mm) maintained at room temp and wavelength 220 nm. The mobile phase consists of buffer (potassium dihydrogen pH 4.0) and methanol (65:35 v/v). The flow rate is maintained at 1.0 mL/min. The calibration curve was linear and regression coefficient (R2) value was found to be 0.999 and concentration ranging from 1.5-4.5 µg/mL and 100-300 µg/mL for Ertugliflozin and Metformin hydrochloride respectively. The LOD and LOQ of the method were found 1.04 μg/mL, 9.61 µg/mL and 0.0007 µg/mL, 0.006 µg/mL for Ertugliflozin and Metformin HCl. The developed method was found to be simple, precise, specific, linear and accurate as validated as per USP and International Conference on Harmonization (ICH)...
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Posted by admin on Dec 31, 2019 in |
Arnebia benthamii (synonym – Microtomia benthamii) commonly known as Ratanjot or Laljari found in western Himalayan region and used as wound healer roots by local vaids and tribes. The roots of Arnebia benthamii contain a red dye that is used in various health disorders like fungal infection, inflammation, fever and coloring/flavoring agent in Indian curries. To estimate wound healing potential of root extract of Arnebia benthamii, percentage wound contraction, wound area, epithelization time, tensile strength and wound index were measured in 13 days of wound healing study. The results showed highly significant wound healing in excision as well as incision wound healing models (P<0.01). The Arnebia benthamii extract-treated wounds showed about 41% higher wound contraction rate and 44% increased tensile strength in comparison with negative control animals. The epithelization time decreased by 43% and wound index value decreased by 89% in comparison to negative control animals which indicated that methanolic root extract of Arnebia benthamii is very helpful in faster and high-quality wound healing (P <...
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