Posted by admin on Jun 30, 2020 in |
This review enumerates the classification, role, and importance of carbohydrate derivatives and glycomimetics. Carbohy-drates are polyhydroxy aldehydes or ketones. Carbohydrates are the most abundant dietary source of energy for all organisms. They are precursors for many organic compounds such as fatty acids and amino acids. They can participate in the structure of cell membranes and cellular functions such as cell growth, adhesion, and fertilization. Glycomimetics are the compounds of low molecular weight based on the structure of functional carbohydrates. The rational style of tiny molecule glycomimetics that exhibit improved drug-like properties like enlarged affinity, blood serum half-life, stability, and bioavailability. Currently, two successful drugs for influenza (Tamiflu, Relenza) are mimicking (Glycomimetics) the transition state of the enzymatic cleavage of the terminal N-acetyl neuraminic acid. A hopeful example is the antibody 2G12, which has been shown to neutralize HIV infectivity. The functional carbohydrates identified in these recognition processes themselves do not make good drug candidates. Rather, their bioactive conformations in their receptor sites can be empirically determined by physicochemical methods...
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Posted by admin on Jun 30, 2020 in |
The aim of this study was to analyze the molecular weight of protein and in-vitro antioxidant activity of marine gastropods Turbo brunneus, Cypraea annulus, and Babylonia spirata. The molecular weight of the protein was determined using SDS-PAGE, and its antioxidant potential was carried out by DPPH radical scavenging activity, reducing power activity and H2O2 radical scavenging activity. The molecular weight of protein varies from 54 kDa in T. brunneus, 38 kDa to 60 kDa in C. annulus, and 44 kDa to 116 kDa in B. spirata. The DPPH scavenging effect was high in Cypraea annulus (78.30%) followed by Babylonia spirata (65.20%) and Turbo brunneus (64.89%). C. annulus showed the highest reducing power of 95.36% at 500 µg/ml concentrations and lowest reducing the power of 52.07% at 100 µg/ml concentrations in the T. brunneus. The maximum radical scavenging activity was reported in T. brunneus (71% at 150 µg/ml concentrations), and minimum activity was reported in C. annulus (8.34% at 50 µg/ml concentration). The results show that tissue extracts of three...
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Posted by admin on Jun 30, 2020 in |
The objective of current research work is exploring the use of natural mucilage for sustained release characteristics for the formulation of microspheres. Orifice ionic gelation method was used to formulate the microspheres by using complex-forming polymer sodium alginate in combination with isolated mucilage. Mucilage from Colocacia esculenta corms were isolated and combined with sodium alginate to fabricate in the form of microspheres. Resultant batches were characterized by differential scanning calorimetry, Fourier transforms infrared spectroscopy, scanning electron microscopy, X-ray diffractometry, swelling capacity, flow properties, particle size, and in-vitro dissolution behavior. Isolated mucilage was found to be swellable in water and amorphous in nature. FTIR and DSC study indicates compatibility between drug and selected polymer. All the formulations exhibit better flow properties. Particle size was found in the range of 780-880 micron. The optimized formulation is releasing the drug for the period of 12 h. Colocacia esculenta corms mucilage along with sodium alginate can be efficiently utilized to retard the drug release and minimize the side effects of the drug, so...
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Posted by admin on Jun 30, 2020 in |
Pseudomonas aeruginosa is one of the most emerging multidrug-resistant opportunistic pathogens, which poses a threat to immunocompromised patients. Treatment of P. aeruginosa in the affected patients has become difficult because of its ability to develop resistant strains against most of the conventional therapeutic antibiotics. Quorum sensing plays a crucial role in the virulence nature of P. aeruginosa as it helps the bacteria to produce virulence factors and biofilm formation. In the present study, we studied the quorum quenching potential of fungal secondary metabolites from the well-known fungus Phoma multirostrata PUTY3 strain isolated from Carica papaya. In this study, we have carried out both in-vitro and in-silico analyses of fungal secondary metabolites from P. multrirostrata against P. aeruginasa. The in-vitro analysis showed a marked decrease in the production of virulence factors such as pyocyanin, rhamnolipids, protease, elastase, exopolysaccharides, HCN gas production and biofilm formation. In addition to this, a significant inhibition in the motility factors of P. aeruginosa such as swimming, swarming, twitching was also observed. In-silico studies further validated...
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Posted by admin on Jun 30, 2020 in |
Nanotechnology is going to revolutionize the world. According to the National Nanotechnology Initiative (NNI), nanotechnology refers to the study of all particles in the range of 100 nanometers or less. One of the most important advantages of the smaller particle size is the ratio of surface atoms or molecules to the total number increases. That means they have large surface areas that lead to increase in surface activity and produce changes in their physical properties and biological properties. In the pharma-world, the applications of nanotechnology mean drugs containing nano-sized active ingredients. They are well used to cure HIV, Cancer and other harmful diseases; they can restore lost tissue at the cellular level, useful for monitoring, diagnosing and fighting sickness. The smaller drug delivery systems permit the deposition of medications in previously inaccessible areas of the body; it also has great importance in the treatment and diagnosis of certain diseases like cancer. A recent discovery in the field of drug delivery is target therapy, which improves the diagnostic tests and...
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