Posted by admin on Mar 31, 2021 in |
A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was developed and validated for the quantification of lenvatinib in plasma samples. Plasma samples were presented by selecting the Liquid-Liquid extraction (LLE) method with ethyl acetate. The technique was developed and validated with respect to the parameters specificity, sensitivity, carry-over, recovery, precision, matrix effect, accuracy, and stability. The lenvatinib and IS (lenvatinib-D4) were eluted within 3.5 min on Symmetry-C18 column (50 mm × 4.6mm × 5µm) with 0.1% V/V formic acid in acetonitrile, methanol, and 0.1% V/V formic acid in water in the ratio of 70:20:10 as mobile phase with 0.70 ml/min infusion flow rate. This analytical method was also applied successfully to study bio-availability studies in 6 healthy rabbits. LVB was shown Tmax of 3.452 ± 0.754, mean Cmax of 89 ± 3.045, AUC0t of 1700.01 ± 257.892, and AUC0t of 3665.3 ± 259.59 for Test formulation. The method can be useful in the quantification of lenvatinib in clinical research organizations for bioavailability studies and in forensic...
Read More
Posted by admin on Mar 31, 2021 in |
Ruellia tuberosa, commonly called Minnie root, has plenty of applications in herbal medicine due to the presence of several bioactive compounds. A simple, efficient, and reproducible in-vitro plant regeneration protocol was developed for Ruellia tuberosa. The cotyledonary nodal explants were excised from seedlings were inoculated on Murashige and Skoog (MS) medium supplemented with various concentrations of BAP and NAA, respectively. The highest numbers of 9.86 ± 0.15 shoots/explant were obtained in MS medium augmented with BAP (2.0 mg L-1) and NAA (1.0 mg L-1). The micro shoots induced per explant significantly increased with the supplementation of BAP to auxin-containing medium. The results showed that maximum percentage of multiple micro shoots 89%, with a number (13.80 ± 0.26) shoots / explant was found on MS medium supplemented with BAP (2.0 mg L-1) and NAA (1.5 mg L-1). Further, the highest frequency of micro shoots was produced on MS medium supplemented with TDZ (0.3 mg L-1) and AgNO3 (0.4 mg L-1) for large-scale production. The highest frequency of root induction (96%)...
Read More
Posted by admin on Mar 31, 2021 in |
The World Health Organization (WHO) declared COVID -19 a pandemic on March 11 2020. As of today, there have been 122 million COVID -19-update cases worldwide, and the counting goes on. Unfortunately, strategic measures such as total lockdown, social and self-isolation, and gradual lifting of some of these restrictions both pose economic, social, and psychological distress and are thought to have affected people more than the virus itself. There is no specific antiviral drug available at this time. However, there are currently five main vaccines on the market that aim to protect the population from contracting the coronavirus. Moderna, Pfizer/BioNTech, Oxford/AstraZeneca, Sputnil V, and Sinopharm vaccines are among those included. Furthermore, developing an effective medication that is reliable for the treatment may be more effective, but it is more difficult. To accomplish this, molecular bio-interaction, virulence, clinical, and pathological features of COVID -19 viruses must be clearly understood. Additionally, the designation of potential antiviral drugs in the form of nanomedicines will optimize the therapeutic outcome and improve the quality of...
Read More
Posted by admin on Mar 31, 2021 in |
Biosimilars are the most promising medicines for treating complex diseases. The first biosimilar was approved in India in 2000, and since then, there is no looking back. CDSCO in collaboration with DBT has developed new guidelines in 2012, which were revised in 2016 for pre and post-marketing approval of biosimilars in India. India is one of the leading manufacturers of biosimilars catering to domestic and international market. Soon it will have a vast opportunity to develop more similar biologics as the patent of many biologics is scheduled to expire by the year 2020. India has emerged well in global market of biosimilars. India started its journey with near about US$ 250 million biosimilar market in 2015 and it is estimated to reach around US$ 40 billion in India while US$ 240 billion in world by 2030. Biosimilars are the boon in treating...
Read More
Posted by admin on Mar 31, 2021 in |
Coronaviruses are a group of viruses that belongs to the Coronaviridae family. These viruses have helical symmetry, enveloped, positive-sense, single-stranded RNA genome with nucleocapsid that causes diseases in birds and mammals. SARS-CoV 2 complete genome has genome size of 29,903 bp, having nine genes identified as Orf1a Polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF7a Protein, ORF8 Protein, Envelope proteins, Spike Surface glycoprotein, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. Based on the ProtParam result, ORF1a has more molecular weight (489989 daltons), and Envelope protein has a less molecular weight (8365 daltons). The pI is lower in Orf1a polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF8 Protein, and Spike Surface glycoprotein) and high in ORF7a Protein, Envelope proteins, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. The Orf1a Poly-protein, Orf1ab Polyprotein, ORF3a Protein, Envelope proteins, Spike Surface, and Membrane glycoprotein are stable. The higher aliphatic index and more hydrophobicity of membrane glycoprotein suggest an increase in the thermostability of protein might favor an increase in its solubility. The vaccines like EIAV vaccine gp45, anti-SARS m396 Antibody, EIAV vaccine...
Read More
