Posted by admin on Mar 31, 2021 in |
Objective: Ethyl para toluene sulfonate is a genotoxic impurity and the current work’s primary objective is to develop a hyphenated and highly sensitive LC-MS method for the determination (EPTS) in Perindopril tert-butylamine. Method: EPTS was determined by LC-MS method in selective ion monitoring mode (SIM) using Atlantis T3 (150×4.6mm) 3.0 µm column. 0.01M ammonium acetate is utilized as buffer. Gradient elution was employed and the Mobile phase A is composed of mixture of buffer and methanol in 70:30 (v/v) ratio along with Mobile phase B made up of buffer and methanol in 5:95 (v/v) ratio. The gradient program (T/%B) was set as 0/5, 2.50/5, 7.00/50, 12.00/70, 18.00/95, 21.00/95, 24.00/5, 30.0/5. Results: The LOD and LOQ values found for EPTS were 0.018 µg/mL and 0.054 µg/mL. The method has accuracy within 98.1-103.5% for the analyte. Developed method validated as per International Conference on Harmonization guidelines. Conclusion: Developed LC-MS method in SIM mode can be applied as a good quality control tool for quantitation of EPTS impurity at very low levels...
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Posted by admin on Mar 31, 2021 in |
A new stability indicating Ultra-Fast Liquid Chromatography (UFLC) method was validated for the simultaneous estimation of Metformin hydrochloride (MET) and Vildagliptin (VILDA) in bulk and tablet dosage form as per the International Council for Harmonization guidelines. Chromatography developed on Shimatzu UPLC, X-Bridge C18 column equipped with PDA detector. Mobile phase composed of phosphate buffer (25 mM) pH 3.0: acetonitrile: methanol (85:13:2). Sodium 1-octanesulfonate monohydrate (0.25 g/L) added in aqueous buffer. The effluent was passing through the column 1.0 ml/min at 40 ºC. The retention time of MET and VILDA were found to be 2.964 min and 6.358 min. The specificity study was determined by the addition of known impurities 1-cynoguanidine and vildagliptin amide of MET and VILDA, respectively. The forced degradation studies indicated that MET was stable while VILDA was susceptible to alkaline hydrolysis and peroxide oxidation. The degradation product peaks were well resolved from the MET and VILDA peaks. Purity angle and purity threshold of the MET and VILDA peak showed spectral homogeneity over the entire peak region...
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Posted by admin on Mar 31, 2021 in |
There has been an exponential growth in plant-based products during the last few decades for the treatment of various ailments. According to literature, about 25% of prescribed drugs are of plant origin globally. One such important plant is Asystasia gangetica, also known as ‘Chinese violet’ or ‘Ganges primrose’ is an ornamental, scattering, groundcover herb. The plant is about 0.30 to 0.60 m in height, mainly distributed in sub-Saharan tropical Africa, Arabia, and tropical Asia (including India). Ethnobotanically, the decoction of its leaves is effective in the treatment of rheumatism, stomachache, anthelmintic, heart pains, asthma, astringent, diaphoretic, woman infertility, etc. It is mainly reported to contain chalcone, biflavons, glucoside, amino acid, iridoid glycoside, etc. Pharmacologically plant is reported to possess anti-asthmatic, anti-hypertensive, anti-diabetic, anti-hyperlipidaemic, anti-microbial, anti-oxidant activity, anti-snake venom, etc. The present review is an attempt to describe the important medicinal properties of A. gangeticain the traditional and modern scientific research. It is concluded that the plant has numerous therapeutic potential which need to be...
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Posted by admin on Mar 31, 2021 in |
Now a day’s interest towards natural and has been growing due to the unhealthy consequences of chemicals in the health industry; though, herbal substances possess several quality control and confirm pharmacological action issues. This present study was designed to determine the effects of Salix alba L. methanolic extract (MESAL) for its antioxidant and anti-inflammatory activity in rat models. Further to establish and validate a sensitive, fast and reproducible high performance thin layer chromatographic (HPTLC) method of two biomarker compounds Salicin and Ferulic acid from MESAL. The anti-inflammatory activity was studied by the carrageenan induced rat paw oedema method while DPPH free radical scavenging ability was utilized to determine the antioxidant activity. Additionally, the separation was performed by HPTLC with quantification of markers (Salicin and Ferulic acid). Among the different combinations of mobile phases used, best separation was achieved in Toluene: Ethyl acetate: Methanol: Formic Acid (5:3:1:1v/v/v/v). The MESAL exhibited antioxidant activity with a maximal inhibitory concentration (IC50) value of 400 µg/ml and exerted anti-inflammatory activity, wherein 70% protection was...
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Posted by admin on Mar 31, 2021 in |
A simple UV spectrophotometric method using the first order derivative technique was developed for the simultaneous estimation of tacrolimus and berberine hydrochloride in nanoparticles. The tacrolimus and berberine hydrochloride stock solutions were prepared methanol and scanned in UV- region for first-order derivative spectrum. The zero-crossing point for tacrolimus and berberine hydrochloride was 369 nm and 263 nm, respectively. Linearity was established over the concentration range of 0.5-2.5 μg/ml and 5-25 μg/ml for tacrolimus and berberine hydrochloride with correlation coefficient (r2) value 0.9933 and 0.9796respectively. The method was validated according to ICH guidelines for validation parameters like accuracy, precision, the limit of detection, and the limit of quantification. They found to be within limits. The method was successfully applied for the quantitative estimation of tacrolimus and berberine hydrochloride in...
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