Posted by admin on Jan 31, 2021 in |
Studies of natural products emerge as an important medicinal resource. An example is the use of Zanthoxylum chiloperone (Rutaceae) for medicinal purposes against neglected diseases, as for example, Chagas disease and leishmaniasis. The analysis of the chemical composition of this medicinal plant was carried out by chromatrographic techniques to evaluate its pharmacological applications. The use of factorial design is currently the most common optimization technique used in the design of chromatographic experiments. We proposed the optimization and validation of the analytical methods, working with high-performance liquid chromatographic coupled with array diode array detection (HPLC-DAD), and finally, we developed the first stability test of natural 5-methoxycanthin-6-one (5-MCT). The establishment of the analytical methodology was performed using a full factorial design. The quantitative determination of 5-MCT was validated with the parameters of the factorial design: temperature of 25 ºC, methanol concentration of 70%, and flow of 0,8 mL/min. Preliminary stability tests showed that some conditions presented significant reductions of 5-MCT, and the respective equations were formulated. Ten related degradation products were...
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Posted by admin on Jan 31, 2021 in |
Evaluation of nootropic activity of Semecarpus anacardium leaves ethyl alcohol extract on exteroceptive behavior and interoceptive behavior was investigated by use of Shuttle box, Continuous Avoidance Response Apparatus and Elevated plus-maze test, Scopolamine-induced amnesia, Diazepam-induced amnesia, and Sodium nitrite-induced amnesia. The beha-vioral study was done using Lithium- induced head twitches (5-HT mediated behavior) test. The ethyl alcohol leaves extract was administered orally in two different dosages to male albino rats (100 and 200 mg/kg daily, p.o. & isolated flavonoid 40 mg/kg daily p.o) and Swiss albino mice (100 and 200 mg/kg Daily p.o. & isolated flavonoid 40mg/kg daily p.o). The result was compared to piracetam (100 mg/kg, p.o) used as a standard drug. The buzzer (conditioned stimulus) and an electric shock (unconditioned stimulus, 30 v and 0.5 sec) were used in the Shuttle box. The ethyl alcohol extract of Semecarpus anacardium leaves showed statically significant improvement in memory retention and learning when compared to control. The study shows that the ethyl alcohol extract of Semecarpus anacardium leaves has the...
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Posted by admin on Jan 31, 2021 in |
The validated stability-indicating analytical method was developed for quantification of Atazanavir using Atazanavir-D9 as an internal standard by UPLC-ESI-MS/MS. The method was validated according to United States Pharmacopeia (USP) guideline with respect to the accuracy, precision, specificity, linearity, solution stability, robustness, sensitivity, and system suitability. Forced degradation study was validated according to International Conference on Harmonisation (ICH). For this, an isocratic condition of mobile phase comprising 10 mM Ammonium Acetate Buffer (pH: 4.0): Methanol: Acetonitrile, (20:40:40%, v/v/v) using the CORTECS C18, 90Å, 2.7 µm, 4.6 mm × 150mm. The total analysis time was 4.0 min, and the flow rate was set to 0.5 ml/min. The mass transitions of Atazanavir and Atazanavir-D9 obtained were m/z 705.3 × 167.9 and 714.9®167.9. The standard curve shows a correlation coefficient (r2) greater than 0.999 with a range of 150.0-450.0 pg/ml using the linear regression model. Forced degradation of the drug product was carried out as per the ICH guidelines with a view to establishing the stability-indicating property of this method and providing useful...
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Posted by admin on Jan 31, 2021 in |
Ozone gas (O3), being an unstable molecule, attracts uncertainty as an alternative to medication, opening avenues for copious research. Ozone therapy (OT) shows its therapeutic efficacy by its reactions with biological components and the production of oxidative stress. It is found useful in various disease conditions like cancer, cardiovascular diseases, pulmonary diseases, wound healing, dental conditions, etc. The therapy on contrast also shows side effects depending on the type of treatment includes burns, coughing, cramping, headaches, nausea, vomiting or severe respiratory complications, and also sometimes flu-like symptoms. The investigation for the use of the OT as a technology is still under development to be used in several ways like ozonated water, injections, insufflations, oil, blow the gas into the body, use an ozone sauna, autohemotherapy. The therapy raises many questions to be used as a solution for diseases with self-contradiction due to the adverse effects occurring after the use of therapy or exposure to the...
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Posted by admin on Jan 31, 2021 in |
The study aimed to enhance the solubility and develop a polymer (Guar Gum/Xanthan gum) based colon targeted drug delivery system of Quercetin promising in-vitro mouth-to-colon release profile. The solubility of the drug was enhanced by the solid dispersion method using solvent-evaporation technique; the optimized formulation 1:5 (Quercetin: PVP) was targeted to colon. Thermal studies proved that the drug is in an amorphous state. FTIR studies indicated no interaction between the drug and the polymers. The in-vitro drug release studies indicated that among all, F5 and F6 type formulations were promising to target the colon with a release of 96.30% at the end of 24th h. F6 formulation exhibited a release of 90.12% at the end of 24th h. The release kinetics was found to follow zero-order by super case- II transport. Simulated dissolution studies of the optimized formulations using 4% rat caecal demonstrated a maximum release of 100.36 & 98.75% at the end of 24th h in colonic contents. The tablets showed a release of 92.78± 0.61 at the...
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