Posted by admin on May 31, 2021 in |
A simple, sensitive, and fast single-step extraction method for estimating empagliflozin in human plasma using LC-MS/MS was developed and validated for pharmacokinetics evaluations. Plasma samples were basified before solid-phase extraction on SOLA (30 mg / 1 mL cartridges). Separations were carried out on a normal reverse phase C18 column (Hypersil BDS 100 × 4.6, 5µ mm column) for 3.5 minutes at a flow rate of 0.6 mL/min. Ten µL of the SPE eluent is directly injected onto LC-MS/MS to quantify the analyte from 1.563-800.000 ng/mL using a single SRM transition (m/z: 449.140à371.100) in negative ion mode. During method validation, selectivity, matrix effect, recovery, carry-over effect, stability studies, inter-day, and intra-day precision and accuracy experiments were conducted per USFDA guidelines. Method validation data has successfully met the acceptance criteria making it suitable for use in routine bio-analytical laboratories. The scope of this assay can be extended to cover the requirement of preclinical, toxicology, and PK/PD...
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Posted by admin on May 31, 2021 in |
Cadaba indica Lam (Indian Cadaba), an Indian traditional medicinal plant has been used for various diseases. The cold macerated methanolic leaf extract of Cadaba indica was assessed using Agilent GC 7890A gas chromatography connected with an MS- 5975C mass spectrometer and the mass spectra were matched with NIST 14.0 – data library. Several chemical constituents were identified within 28 min of the entire GC-MS analysis. The GC chromatogram shows that presence of most abundant linolenic acids and its esters such as Hexa-decanoic acid, methyl ester, n-hexadecanoic acid, 10,13-octadecadienoic acid, methyl ester, 9, 12, 15-Octadecatrienoic acid, (Z, Z)-methyl ester, Octadecanoic acid, methyl ester, 9, 12-Octadecadienoic acid, (Z, Z)-, 9, 12, 15-Octadectatrionic acid, (Z, Z, Z)-,Octadecanoic acid, Hexadecanoic acid, 2,3-dihydroxy propyl ester and 9- Octadecanoic acid, (Z) – methyl ester. In contrast, compounds with diterpenes, phthalic esters, pyrrolidine, phenol, ketones, and myristic acid derivatives were also detected with average peak area percentage. The nature and chemical background of the detected phytochemical constituents may be responsible for the pharmacological effects of Cadaba...
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Posted by admin on May 31, 2021 in |
Aim: The present study was to develop and evaluate antiulcer polyherbal tablet as designing of oral herbal formulation. The methanolic extracts of Abrus precatorius (MEAP) and Cordia wallichi (MECW) leaves were used in different concentrations in the formulations. Materials and Method: The current exploration was to design a gastro-retentive drug delivery system of MEAP and MECW using swelling polymer through the wet granulation method. All the formulations were evaluated for weight variation, hardness, friability, drug content, and in-vitro dissolution. In this gastro-retentive dosage form using hydroxypropyl methylcellulose-K4M was prepared to develop sustain release tablets, which could remain in the stomach for longer periods of time, delivering the drug to the site of action. Results: Pre and Post-compression parameters of all the formulations were within the Pharmacopoeial limits, and in-vitro drug release of F8 formulation was found to be 98.29% in 12 h. Conclusion: Dissolution studies of the composition, it was concluded that the formulation F8, which is containing 200 mg of polyherbal extracts, 140 mg of HPMC-K4M, 85 mg...
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Posted by admin on May 31, 2021 in |
An amyotrophic lateral sclerosis is a group of progressive neurodegenerative disorders of motor neurons that leads to weakness, muscle atrophy, progressive paralysis, and respiratory insufficiency with a life anticipation of only three years after the onset of disease symptoms. It impairs the functions of both upper and lower motor neurons. The specific mechanism for the disease remains largely unidentified. At present, there is no specific treatment to cure or reverse the progression of the disease. ALS is generally diagnosed based upon the medical history and signs and symptoms; however, it is quite difficult to diagnose the disease in its initial stages. Several studies indicate that various predominant disease mechanisms such as oxidative stress, excitotoxicity, mitochondria dysfunction, aberrant protein homeostasis, defective RNA processing, formation of cytoplasmic inclusion, endoplasmic reticulum stress, endosomal dynamic dysfunctions, protein aggregation, neuroinflammation, oligodendrocyte degeneration, and microglial activation are involved in the pathophysiology of ALS. The various gene mutations (c9orf72, TARDBP, SOD1, and FUS) are also associated with disease mechanisms, so ALS is a complex genetic disorder...
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Posted by admin on May 31, 2021 in |
The objective of the present study was to improve the oral bioavailability using high-density gastro-retentive pellets containing solid dispersion of acyclovir. Solid dispersion prepared with polyvinylpyrrolidone (PVP K30) and hydroxypropyl methylcellulose (HPMC) using different ratios 1:1, 1:2, 1:3, 1:4, and 1:5 by solvent evaporation method and characterized for solubility study, dissolution study, FT-IR, DSC, and XRD. High-density gastro-retentive pellets containing solid dispersion of acyclovir was prepared by extrusion/spheronization technique using solid dispersion of acyclovir with PVP K30 (1:3), Barium sulphate as high-density material, microcrystalline cellulose (MCC) as extrusion aid, ethylcellulose (EC) as release retarding polymer and HPMC as swellable gel-forming polymer. The formulation was optimized based on in-vitro release profile and characterized for pellets morphology, micro-meritics properties, FT-IR, DSC, XRD, and in-vivo study. The optimized formulation F8f showed the release for 12 h with an increase in retention at the absorption site. Release kinetic studies of optimized formulation showed that the data best fit in Higuchi’s model. The in-vivo studies like pharmacokinetic parameters and X-ray transmission were investigated in...
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