Posted by admin on May 31, 2021 in |
A new, simple, rapid, accurate, and economical method has been developed for the simultaneous estimation of Lamivudine and Tenofovir Disoproxil Fumarate in formulation by using a quality by design approach. Design expert software was used for QbD analysis. 03 level factorial quadratic design models were used to analyze the response. The model generated was found to be significant. The optimized conditions of factors were concentration 10ug/ml and wavelength (λmax) 272 nm for LAM and 259 nm for TDF. The distilled water was used as a solvent for analysis. The linearity was observed in the concentration range of 02-100 µg/ml for Lamivudine and Tenofovir Disoproxil Fumarate, both drugs. The simultaneous equation method was used for estimation, and the method was validated as per ICH guidelines. The recovery of Lamivudine and Tenofovir Disoproxil Fumarate was found in the range of 98.90-100.77% and 101.63-102.43%. The stability testing was done as per ICH guidelines. The developed method may be used by industries for analyzing their products....
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Posted by admin on May 31, 2021 in |
Cathepsin S enzyme has been considered as an evolving target for the development of novel therapeutic agents for the treatment of numerous autoimmune disorders and other inflammatory diseases. Using TSAR 3.3 2D QSAR has been performed on a series of dipeptide nitrile nucleus. Attempts have been made to derive and comprehend a correlation between biological activity and the generated descriptors. The study was carried out using 37 compounds by division into training and test set by a random selection method. A final QSAR model was generated from a set of 28 compounds with the Leave-out one row (LOO) method of cross-validation to estimate the model’s predictive ability. The most significant model with n = 28, r = 0.969, r2 = 0.939, r2cv = 0.801, s value = 0.35, f value = 89.07 was developed using MLR analysis. For PLS, the fraction of variance explained = 0.928 was observed. A comparable PLS model with r2 = 0.928 and Neural model with r2 = 0.962 indicated good internal predictability of the...
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Posted by admin on May 31, 2021 in |
Brahmi (Bacopa monnieri Linn.) is a miraculous Ayurvedic drug used commercially in memory-enhancing formulations. Its nootropic action is due to Bacoside A, which modern parameters have proved. Bacoside A content fluctuated during the course of the whole year. In the present study, High-Performance Liquid Chromatography was conducted to evaluate total Bacoside A content (expressed as the sum of Bacoside A3, Bacopaside II, Jujubogenin and Bacopasaponin C) on a monthly basis in methanolic extract of Bacopa monnieri collected from the wild and cultivated area during Summer, Rainy and Winter season of a year. Instead of the entire herb, 10 cm apex part and the remaining part of Brahmi were used. Chromatographic analysis was performed with Shimadzu Prominence HPLC instrument; equipped with a quaternary pump, LC2010CHT, degasser DGU-20A3R, Column oven CTO-10As, Autosampler SIL-20 HT, Diode-Array-Detector SPD-M20A with Prime SIL C18 column. Among individual plant parts, maximum Bacoside A content was observed in the remaining part of a plant in July for the wild sample (38.86%) and in the remaining part of...
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Posted by admin on May 31, 2021 in |
Objective: To assess the response of Preterm neonates to Tactile and Kinesthetic Stimulation. Methods: The study was conducted in a Neonatal Intensive Care Unit of a tertiary care hospital in India. Twelve stable preterm neonates were selected as per the inclusion and exclusion criteria and randomized into the intervention and control group. Seven days of tactile and kinesthetic stimulation was given three times in a day to intervention group along with the routine care, and the control group was given only the routine care. The pre and post-test assessment was done among both the groups. The weight gain and other physiobehavioural parameters were compared with the control group. Result: The preterm neonates in the intervention group gained more weight than the control group, and also there was a significant difference found in the physio-behavioural parameters between the two groups. Conclusion: Tactile and kinesthetic stimulation is a non-invasive cost-effective intervention that promotes weight gain and reduces hospital stay. Hence it can be recommended to the preterm neonates in the Neonatal...
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Posted by admin on May 31, 2021 in |
This study aims to identify the suitable method for extraction of polyphenols from Acacia leucophloea bark using enzymes and to optimize the processing condition for extraction using Response Surface Methodology. A microwave-assisted enzymatic extraction (MAEE) method was developed and optimized to augment the polyphenol yield. The optimal extraction conditions were as follows: the amount of cellulase 1%, agitation speed 108 rpm, incubation time 1.5 h, and irradiation time 6 min. Under these conditions, polyphenol yield could reach 6.31%, which was higher than other extraction methods studied. The antioxidant activity of the crude extract and the encapsulated extract was evaluated by DPPH radical and reducing power assay. The antibacterial activities of crude extract and the encapsulated extract were studied using the disc diffusion technique. Among the tested bacteria, Klebsiella pneumoniae was most susceptible to polyphenol extract with the highest inhibition zone of 18.6 ± 0.5 mm at the concentration of 8 mg/mL. The outcome of the study indicates that the MAEE method was efficient and eco-friendly, and the polyphenols have...
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