Posted by admin on Apr 30, 2021 in |
A simple stability-indicating high-performance thin-layer chromatographic method which is economic, selective, and precise for analysis of Sofosbuvir (SFB), both as a bulk drug and in formulations, was developed and validated according to ICH guidelines. The method employed HPTLC precoated Merck TLC plates RP-18 F254 as the stationary phase while the solvent system prepared by mixing n-Hexane: Ethyl Acetate: Methanol in proportion 5:3:2 v/v. The system was found to give a compact spot for the drug (Rf value of 0.452 ± 0.004). Densitometric analysis of SFB was carried out in the absorbance mode at 261 nm. The linear regression analysis data for the calibration plots showed a good linear relationship, R2 = 0.9994, with respect to peak area in the concentration range 100-600 ng/band. The LOD and LOQ were 8.009 ng/band and 24.270 ng/band, respectively. SFB was subjected to hydrolysis, oxidation, and thermal degradation, which indicates the drug is susceptible to hydrolysis, oxidation, and heat. The method was validated for precision, recovery, and robustness. Statistical analysis proves that the method...
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Posted by admin on Apr 30, 2021 in |
Antimicrobial activity of the various species of Cymbopogon plants like Cymbopogon citrates, Cymbopogon martini, Cymbopogon nardus, Cymbopogon flexuosus, Cymbopogon schoenanthus against various pathogens like E. coli, Bacillus subtilis, Streptococcus species, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Serratia marcescens were studied with the help of agar well diffusion method. The samples were extracted with solvents like Methanol, Ethanol, Chloroform, Acetone, Diethyl ether to measure the antimicrobial activity by using Muller-Hinton agar plates. The Chloroform and Diethyl ether extract of Cymbopogon flexuosus, Cymbopogon martini showed the highest zone of inhibition against Staphylococcus aureus and Pseudomonas aeruginosa. In Cymbopogon citrates, all the pathogens were resistant to all the extracts except for Streptococcus species and Pseudomonas aeruginosa, which showed the least susceptibility to all the extracts except for Methanol and Diethyl ether extract. In Cymbopogon schoenanthus, Methanol extract showed the highest zone of inhibition against Staphyloccocus aureus and Bacillus subtilis, but Klebsiella pneumonia showed resistance to all the extracts except for Diethyl ether which showed the least zone of inhibition. In...
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Posted by admin on Apr 30, 2021 in |
Cadmium can cause various health problems even at low concentrations by inducing oxidative damage in tissues of organisms. Nowadays, the focus has been raised toward the use of herbal treatment against heavy metal toxicity. Hence, the present research work was aimed to investigate the protective effect of curcumin against Cd-induced toxicity in the auricles and ventricles of the heart in albino mice. Mice were equally divided into 8 groups, with 5 mice in every group. The experiment was performed in two intervals of for 15 and 45 days. Mice were divided into the following groups: control, CdCl2 (1mg/kg bw of Cd daily), CdCl2+Curcumin (1mg/kg bw of Cd daily+100 mg/kg bw of Curcumin on alternate days), and Curcumin (100 mg/kg bw of Curcumin on alternate days) and then marked as group 1, 2, 3 and 4 for 15 days and group 5, 6, 7 and 8 for 45 days interval. Animals were sacrificed after 15 and 45 days of treatment. The biochemical analysis depicts the generation of oxidative stress with...
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Posted by admin on Apr 30, 2021 in |
The dry ginger rhizome was extracted with ethyl acetate, and gingerol content was enriched by liquid-liquid extraction. The extract was analyzed for the percentage of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol by HPLC. The acute toxicity was evaluated as per OECD guidelines 423. Ginger extract was fed at 2000 mg/kg body weight to overnight fasted female rats (8-10 weeks old; 160-180 g). The animals were observed daily for clinical signs of abnormality/mortality. After 14 days, animals were sacrificed, and gross pathological changes were recorded. Sub-acute toxicity of ginger extract was studied by feeding the extract at 100, 500, and 1000 mg/kg daily to rats as per OECD guidelines 407. The total gingerols content in the purified viscous extract was 36-43%. The finished formulation of ginger extract used for the toxicity studies had 8% total gingerols. In the acute toxicity study, no mortality or clinical signs of toxicity were observed at a maximum recommended dose level of 2000 mg/kg; therefore, the LD50 is >2000 mg/kg in rats. The repeated administration of...
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Posted by admin on Apr 30, 2021 in |
The present study aimed to determine the phytochemical components of the leaf extracts of A. malabarica, antimicrobial, antioxidant activities and GC-MS analysis. The leaves extracts have folklore and traditional applications in India. The polar and non-polar solvents extracts such as methanol, ethyl acetate, chloroform, hexane and water were used for various phytochemical analysis proved the existence of flavonoids, glycosides, phenols, 3terpenoids, tannins and alkaloids. Similarly, five different extracts of A. malabarica were tested for the antibacterial activities against five human pathogens. The present results revealed that, the different solvent extracts showed maximum zone of inhibition against all the tested strains. The plant extract was highly effective against E. coli and E. aerogenes. In addition, the extracts noted promising GC-MS analysis showed the presence of bioactive...
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