Posted by admin on Mar 31, 2021 in |
The maintenance of an appropriate serum concentration of a drug is important to ensure therapeutic efficacy. Functional alterations of drug transporters may influence the serum concentration of drugs through changes in its pharmacokinetics and pharmacodynamics (PK/PD). In this study, we have focused on the influence of functional alterations in the intestinal P-glycoprotein (P-gp) on the PK of linagliptin (LIN) by the oral route under normal and diabetic conditions before and after pretreatment with resveratrol (RSV) and verapamil (VER) as standard. The interaction between LIN and RSV has been studied using in-vitro noneverted sac study, in-situ Single-Pass Intestinal Perfusion (SPIP) study, and in-vivo oral bioavailability study in rats. Pretreatment with RSV significantly enhanced the intestinal transport, apparent permeability, and effective permeability of LIN in normal and diabetic rats indicating the active role of P-gp in LIN absorption. Pretreatment with RSV significantly altered the Cmax and AUC of LIN in both normal and diabetic rats. But compared to normal rats, diabetic rats show a significant decrease in intestinal transport, and increased...
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Posted by admin on Mar 31, 2021 in |
Objective: In the present work, A Simple, rapid, sensitive, precise, and reproducible, specific UV spectrophotometric method for the determination of Saxagliptin (SAXA) and Dapagliflozin (DAPI) in bulk drug and pharmaceutical dosage form were developed and validated. Methods: A simple double beam UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, the limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. Results: UV-visible spectrophotometric method, measurement of absorption at a maximum wavelength in 10 ml methanol and volume make with water solvent system as reference SAXA and DAPI were found to be at 224 nm and 274 nm respectively. The drug obeyed the Beer’s law and showed a good correlation. Beer’s law was obeyed in the concentration range 2-10 µg/ml for Saxagliptin and 4-20 µg/ml for Dapagliflozin, respectively with a correlation coefficient was 0.999. The LOD and LOQ of Saxagliptin were found to be 0.040 μg/ml and 0.01230 μg/ml, Dapagliflozin was found to be 0.1230 μg/ml and 0.5460 μg/ml,...
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Posted by admin on Mar 31, 2021 in |
Tuberculosis is one of the major contagious diseases and a leading cause of death in humans. Current treatment methods suffer from a multitude of problems like long duration of treatment, lack of patient compliance, presence of drug-resistant bacteria, etc. To combat the disease effectively, there is an urgent need for the development of new drugs effective against both drug-sensitive and drug-resistant bacteria. Cynodon dactylon is known to be effective against cough and other lung diseases. The objective of the present study is to identify the component in Cynodon dactylon, which is therapeutically effective against Mycobacterium tuberculosis by in-silico docking analysis using Autodock. Here InhA, the enoyl acyl carrier protein reductase from Mycobacterium tuberculosis was taken as the target protein for docking analysis. Phytochemicals from Cynodon dactylon were selected based on molecular and ADME properties. Among the phytochemicals investigated, luteolin showed a good docking score and better interaction with the target protein and is comparable to the commercially available first-line antitubercular drug, isoniazid. Based on our preliminary analysis of molecular...
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Posted by admin on Mar 31, 2021 in |
Milk is a complex system where analysis of metal ions is a difficult task due to the presence of interfering materials. The formation of a coloured complex between the metal and a coloured or colourless ligand is the basis for the development of spectro-photometric methods. The aim of this study is to analyze and estimate copper present in various samples of milk (i.e., cow, buffalo, goat, camel, soya, and powdered milk) by micellar spectrophotometry method. For this purpose, the ligand N-(2’-Pyridyl)-2-hydroxy-benzamide (NPHB-2) was synthesized by the reaction of 2-aminopyridine with salicylic acid, which is a weak acid and is found to be soluble in alcohol, acetone, and chloroform. It is insoluble in water at a pH less than 7. In addition to the solution of this ligands to copper (II) solution in the presence of micellar medium, a yellowish-green or light green coloured complex is formed immediately. It has been observed that in the presence of a micellar medium, solubility, as well as absorption intensity, has been found to...
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Posted by admin on Mar 31, 2021 in |
As the incidences of Coronavirus (SARS CoV-2) infections have been increasing at a rapid pace, the need for its containment and treatment has taken center stage for numerous research institutions. The similarity of SARS CoV-2 with that of SARS CoV has led to a jumpstart in the research activities on corona-virus on the same. The number of research articles appearing since the time SARS CoV-2 was discovered has skyrocketed. While every research group has been busy in pushing the research information to the next level, hardly any convincing research has come up the effective treatments or therapeutics against the disease. In this study we conduct a bibliographic analysis for SARS CoV, MERS CoV and SARS CoV-2, and statistically compare the incidence of research articles combining along the “treatment” and ‘therapies” as keywords. We attempt to draw trends and conclusions based on the data available on the databases...
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