Posted by admin on Aug 31, 2022 in |
To the best of our knowledge, the factorial design methodology has never been used to estimate Thymoquinone (TQ) using the reverse phase High-Performance Liquid Chromatography (RP-HPLC) method, making our work unique. The study aimed to create, verify and validate a simple stability-indicating analytical technique for quantitative detection of TQ in Ayurvedic formulations, black seed and polymeric nanoformulation using Design-experiment® (DOE) version 12. The technique was developed utilizing a Luna C18 HPLC column with a mobile phase of water: methanol (25:75, v/v), administered isocratic ally at 1 ml/min, at 253 nm. The validation parameters were carried out in compliance with ICH criteria using the Quality by Design (QbD) approach. The devised technique was shown to be specific, linear (R2 > 0.999) across the specified concentration range of 0.5 to 16 µg/ml, with detection and quantification limits of 0.02 and 0.08 µg/ml, respectively. The applicability of the new approach was evaluated using the TQ entrapped polymeric nanoparticle. TQ entrapment efficiency for prepared nanoformulation was found to be 82.87 percent. The experimental...
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Posted by admin on Aug 31, 2022 in |
Natural products can be the alternative of synthetic medicine and antiviral drugs can be potential from natural products. An attempt was made to evaluate the antiviral activity from the extract of Desmodium gangeticum (L.) DC. as a phytomedicine (GanjhuVirR) for the treatment of dengue fever associated with thrombocytopenia. The present study was evaluated acute and subacute toxicity in rat as well as clinical trial in treated and without treated patients who admitted in hospital due to dengue fever associated with thrombocytopenia. The rats were grouped into group 1 (control) and 2, 3 and 4 (treated) for toxicity test while 51 patients were included in the clinical trial and 25 were treated and 26 provided only standard management as control. The parameters viz. platelet count, fever profile, average hospitalization period, clinical profiles and viral load reduction were tested for both the group. All the data related to morbidity, mortality and behavioural features were observed similar between exposed and control group. The haematological and biochemicals findings were comparable between the group....
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Posted by admin on Aug 31, 2022 in |
Nutraceutical is employed to explain a medicinal or nutritional component with a food, plant, or natural material that will be purified or concentrated. It is used to improve health by preventing or treating disease. Non-alcoholic fatty liver disease (NAFLD) is a clinical condition characterized by lipid infiltration of the liver with a high prevalence in the general population affecting 25% of adults with a double prevalence in diabetic and obese patients. Nearly 1/3 of NAFLD progresses to non-alcoholic steatohepatitis (NASH), leading to liver fibrosis and cirrhosis. There are currently no specific drugs approved on the market to treat NAFLD. So the treatment is essentially based on lifestyle optimization. However, some nutraceuticals may contribute to developing liver lipid infiltration and related anthropometric, haemodynamic and/or biochemical parameters. The objective of this article is to review the available clinical data on the effect of nutraceuticals on NAFLD and other parameters related to NAFLD. There are relatively few nutraceutical molecules that are adequately studied for their effects on NAFLD and other related diseases....
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Posted by admin on Aug 31, 2022 in |
The major pathogenic mechanisms of diabetes are chronic hyperglycemia and glucotoxicity. Excess production of reactive oxygen species due to hyperglycemia results in oxidative stress and changes in mitochondrial energy metabolism promote β cell dysfunction, eventually leading to apoptosis and the development of diabetic complications. Although several oral antihyperglycemic agents are available, their adverse effect profile affects their prolonged use. Medicinal plants, having a low toxicity profile, are being looked at as alternatives for diabetes treatment. The present study was thus planned to elucidate the mechanisms of action of 5 medicinal plantsviz. Azadirachta indica, Momordica charantia, Gymnema sylvestre, Syzygium cumini, and Trigonella foenum graecum selected on the basis of Ayurveda in diabetes using RINm5F cells. The effects of these plants on Streptozotocin (STZ) induced oxidative stress was assessed using variables like lipid peroxidation in terms of malondialdehyde (MDA) release, modulation of apoptosis, cell cycle analysis using flowcytometry, and insulin release using commercially available rat insulin Elisa kits. All the plant extracts in the presence of STZ inhibited the release of...
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Posted by admin on Aug 31, 2022 in |
Antimicrobial activity of stem bark of Mangifera indica was determined using the disk diffusion method to determine the susceptibility or resistivity of Staphylococcus species to the plant extract. Ethanol extract showed the highest inhibition zone diameter of 34mm, while the aqueous extract showed a zone diameter of 23 mm. The ethanol proved to be a better solvent, and the Staphylococcus species was found susceptible to the ethanolic extracts. The stem barks extracts can be used to develop new antimicrobial compounds. Survey results revealed a need to increase awareness to limit the overuse of harmful chemical cosmetics as skin care products and augmentation in the use of organic extract for skincare products among...
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