Posted by admin on Jul 31, 2022 in |
From the primordial times’ plants are standard as a chief resource for mankind. In the traditional system of medicine, Indian sarsaparilla Hemidesmus indicus (Family Asclepiadaceae) is a commonly well-known medicinal plant. This plant contains various phytoconstituents belonging to flavonoids, tannins, glycoside, sterols, and volatile oil. In the traditional system, the plant Hemidesmus indicus is used for loss of voice, cough, menstrual disorders, asthma, abdominal swelling, fever, skin diseases, ulceration due to syphilis and anthelmintic, etc. Aim and Objective: In this review article, we aim to represent an exhaustive update on the ethnobotanical review, phytoconstituents review, pharmacological review, toxicity study, and marketed formula. Materials and Method: Widely used international databases such s PubMed, Scopus, Google search, and JSTOR were searched, and various books like the flora of Orissa, Pharmacology of medicinal plant and natural product, the Indian meteria medica etc. were also searched. Result: Hemidesmus indicus played an important role in traditional Indian medicine. The important pharmacological uses of H. indicus are Anti-inflammatory, Anti-leprotic, Anti-oxidant, Anti-arthitic, Anti-microbial, Anti-diarroheal, woud healing,...
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Posted by admin on Jul 31, 2022 in |
Introduction: The seeds of Aegle marmelos (bael) are less explored for their therapeutic activity; cytotoxic activity of bael seeds was yet to be studied. The present study aims at finding the cytotoxic activity of the successive extracts and the flavonoid fraction from the methanolic extract of the seeds. Methods: The extracts were prepared using solvents (petroleum ether, chloroform, ethyl acetate, methanol, and water) in increasing order of their polarity. Anti-oxidant activity of the extracts was evaluated using DPPH radical scavenging assay, nitric oxide scavenging assay, and hydrogen peroxide scavenging assay. Flavonoid fractions were obtained by thin-layer chromatography of the flavonoid-rich fraction prepared from the total methanolic extract. Human colon cancer cell line (HT 29), human breast cancer cell line (MCF-7), human lung cancer cell line (A-549) were used to evaluate the cytotoxicity of the extracts and the flavonoid fractions. Results: Various phytochemicals like alkaloids, flavonoids, tannins and phenols, steroids, and saponins were detected in phytochemical investigation of the extract. Ethyl acetate fraction showed comparable DPPH radical scavenging activity and...
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Posted by admin on Jul 31, 2022 in |
hERG (human ether-à-go-go-related gene) encodes a potassium ion channel which involved in ventricular repolarization at the end of a cardiac action potential. Mutations in hERG channel result in torsade’s de pointes. The cardiotoxicity is mainly due to the prolongation of the QT interval caused by the blockage of hERG. Indeed, many therapeutic drugs are withdrawn from the market as well as drug candidates may fail in the late phases of drug discovery because of cardiotoxicity. The ICH S7B and ICH E14 are the safety pharmacology guidelines recommended for evaluating new chemical entities. In the early phase of drug discovery, it is important to screen the compound for the activity on the hERG channels. Many hERG assays are available, including Fluorescent Membrane Potential Sensitive Dye Method, Radioactive Ligand Binding Method, Fluorescence Polarization Ligand Binding Method and Rubidium Flux method, Manual Patch-Clamp Method and automated Patch-Clamp Method, Langendorff’s perfused isolated heart method, Non-Invasive Telemetry, and Wireless Telemetry. This review is focused on hERG assay and provides additional information about the long...
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Posted by admin on Jul 31, 2022 in |
Herbal remedies have been utilized in medical practice for thousands of years and are recognized notably as a significant and widely available resource for health treatment. Traditional botanicals and herbal supplements have been deemed to be mild, non-toxic and even safe due to their natural nature. Acacia leucophloea (Roxb.) Willd. belongs to the family Mimosaceae, recognized as a diverse resource of components possessing bioactive qualities. The various parts of Acacia species are used as local treatments for diverse ailments. Especially, the bark is historically utilized for treating several illnesses in the Ayurveda system of medicine. Tanning is found in the bark of Acacia species, and fatty oil is found in the seeds. Analgesic, anti-inflammatory, anti-viral and anti-bacterial properties of A. leucophloea stem bark are used to treat inflammatory conditions such as bronchial inflammation and coughing, biliousness of the skin and leucoderma. Acacia leucophloea has a wide range of potential uses and this review focuses on its pharmacological effects in...
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Posted by admin on Jul 31, 2022 in |
As per the ICH guidelines and regulatory authorities’ worldwide, it has become mandatory to establish a stability-indicating assay method (SIAM) for the drug substance (DS) and drug product (DP) to generate the stability data. It was undertaken to develop a precise, accurate, reliable, rapid, simple and specific method for estimating Teneligliptin free of interference from its probable degradation products. The present investigation has exploited the high-performance liquid chromatography (HPLC) technique. The retention time of Teneligliptin under optimized chromatographic conditions was found to be 5.71 ±0.02 min with a sharp, symmetrical peak (asymmetry of 0.66 ± 0.02). In the system suitability, the drug was found to adequately retain at 5.71 ±0.02 min with a sharp, symmetrical peak and high theoretical plate value of 4808, indicating high column efficiency. The solutions were observed to undergo hydrolysis in 0.1 N HCl and 0.1 N NaOH at room temperature and reflux. Teneligliptin was also found to be susceptible to rapid oxidation. Photolytic stress study indicates that drug in solid-state upon exposure to sunlight...
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