Posted by admin on Feb 28, 2022 in |
A simple, accurate, precise, reproducible, economical UV spectrophotometric method has been developed and validated for simultaneous estimation of Hydroxychloroquine sulfate (HCQ) and Nitazoxanide (NTZ) in a synthetic mixture. Method A by simultaneous equation method, Method B by the first-order derivative method. Method A: simultaneous equation method was based on the measurement of absorbance of nitazoxanide at 345 nm and Hydroxychloroquine Sulfate at 220 nm. Method B: first-order derivative method was based on the measurement of absorbance of HCQ measure at 226 nm (ZCP of NTZ) and absorbance of NTZ measure at 375.50 nm (ZCP of HCQ). The calibration curve was found to be linear in the concentration range 2-10 μg/ml and 5-25μg/ml for HCQ and NTZ, respectively (n=6), with their correlation coefficient of 0.999. The developed method was validated according to the ICH...
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Posted by admin on Feb 28, 2022 in |
The study was designed to study the macroscopic, microscopic, and also physicochemical parameters such as fluorescence analysis, extractive values, ash values, and loss on drying were performed. Therefore, this parameter is essentially used to control the quality of crude drugs and/or herbal drugs/drug products. The moisture content (% LOD) of the leaf powder was found to be 5.10± 0.14 (% w/w), total ash was found to be 12.27 ± 0.3 (% w/w). The amount of acid insoluble ash and water-soluble ash was found to be 14.5 ± 0.25, 6.23± 1.22 (% w/w), respectively. The extractive values of hexane extract were found to be 2.45± 0.26, ethyl acetate extract was found to be5.62±0.17, the water-soluble extractive value of the drug was found to be 9.142 ± 0.58 (% w/w), and the alcohol-soluble extractive value was found to be 14.64 ± 0.28 (% w/w). The results of physicochemical analyses lie within the acceptable limit, which in turn ascertains the quality and purity of leaf...
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Posted by admin on Feb 28, 2022 in |
Objective: To develop a simple, selective, and precise stability-indicating high-performance liquid chromatography method for the simultaneous estimation of acebrophylline and doxofylline in bulk and tablet dosage form. Methods: The chromatographic separation achieved on HiQSil C18 Column (250 × 4.6 mm, 5µm) utilizing a mobile phase Acetonitrile: 10 mM n-hexane sulfonic acid buffer (80: 20, v/v) at a flow rate of 1.0 ml/min with injection volume 20 μl. UV detection was performed at 250 nm. The method was validated as per ICH guidelines. Results: The retention time for acebrophylline and doxofylline was found to be 2.77 min and 9.56 min, respectively. The linear regression analysis data for the calibration plots showed a good linear relationship in the concentration range of 1-10 μg/ml for acebrophylline and 4-24 µg/ml for doxofylline. The percentage recoveries of acebrophylline and doxofylline in the marketed dosage form were found to be 99.91 and 94.24, respectively. The correlation coefficients for acebrophylline and doxofylline were 0.997 and 0.998, respectively. The percentage degradation at different stress conditions like acid,...
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Posted by admin on Feb 28, 2022 in |
Tissue specificity is the essential need of the hour to cut down clinical abnormalities. Allowing desired drug concentration to reach the target site is the current trend of the drug delivery system. Laminin is an active component of the basal lamina and present in most cells and organs has shown great utility in this case. It is a heterotrimeric glycoprotein containing an α-chain, a β-chain, and a γ-chain and found in different isoforms. Laminin influences cell differentiation, migration, adhesion, neurite outgrowth, and angiogenesis. The active peptides screened from the whole-length laminin have been incorporated to develop targeted or tissue-specific approaches. This is because full-length laminin has many active sites and triggers various downstream signaling and functions. Laminin-derived peptides along with various carriers or vectors ensure specific binding to target tissues. The particular therapeutic or diagnostic agent is successfully targeted to the diseased site because of the affinity of peptide sequence towards the cell membrane receptors. Promising applications of laminin-derived peptides are observed in diagnostics, therapy, chemotherapy, and gene therapy....
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Posted by admin on Feb 28, 2022 in |
Studies on dextrocardia have been limited by low numbers. Hence, finding the most common diagnosis in patients with dextrocardia and situs ambiguus is challenging among patients seeking medical attention in a tertiary care center. Aims and objectives: To identify the most common diagnostic pattern in dextrocardia (DC) patients with situs ambiguous (SA). Methods: It is a retrospective study with records dating backto the last 21yrs from a major tertiary care center in South India. In this study, researchers will include all the patients diagnosed with dextrocardia (defined as the right-sided baso-apical axis of the heart). The segmental analysis will be done as described previously. Results: There was a total of n=378 patients with dextrocardia, of which 71 had situs ambiguus with dextrocardia. The mean age was 2.3yrs, 36.6% were females 32 patients had right atrial isomerism (RAI), 18 patients had left atrial isomerism (LAI), and 21 patients had unidentified atrial isomerism (UAI). In the RAI group Double outlet right ventricle (DORV) was present in n=13 (40.6%), Single ventricle (SV)...
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