Posted by admin on Feb 28, 2022 in |
Protein-protein, as well as protein-substrate interactions, play an important role in regulating specific biological functions like signal transduction, apoptosis, gene regulation, and immune response. These play a major role in drug discovery applications and can be used as molecular targets to stop disease development at the molecular level. Thus, understanding the biological mechanism of protein is an integral part of any computational study. Moreover, these interactions can be manipulated through protein engineering to address industrial applications. As part of the protein engineering strategy, the first step is the determination of hotspots. Hotspots are defined as positions in the amino acid sequence that can be targeted for mutagenesis to improve the catalytic activity or stability of an enzyme. The commonly-used method for determining hotspot residues is computational alanine scanning (CAS) mutagenesis experiments, which are computationally expensive and time-consuming. In the current study, we aim to develop a protocol to predict hotspots using a computationally less costly method. We have used two published datasets to cross-validate our method. Both the datasets...
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Posted by admin on Feb 28, 2022 in |
Objective: The Calotropis procera (Ait.) R.Br. (Ascelpidaeaceae) has been used traditionally as anthelmintic, depurative, diaphoretic, emetic, expectorant, febrifuge, and purgative, but no scientific reports are available in the regulation of asthmatic condition. In this present study, Calotropis procera (Ait.) R.Br. was selected to investigate scientifically to establish scientific data for its traditional claim. Materials and Methods: The anti-asthmatic activity of MECP (Methanolic extract of roots of Calotropis procera) and AECP (Aqueous extract of roots of Calotropis procera) was studied by using in-vitro and in-vivo models. The reference drug used was chlorpheniramine (CPM) and Dexamethasone. The in-vitro models included isolated goat tracheal chain and guinea pig ileum preparation. Further, the in-vivo included histamine-induced bronchospasm, milk-induced leukocytosis, passive paw anaphylaxis model, and haloperidol-induced catalepsy. Results: In the in-vitro and in-vivo models, the oral dose of both MECP and AECP showed significant results as compared to control. Among both extracts, MECP showed better activity in the prevention of asthma. Conclusion: Based on phytochemical analysis of the root’s extracts of Calotropis procera (Ait.)...
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Posted by admin on Feb 28, 2022 in |
The current study investigated and compared the antibacterial activity of common kitchen spices; Ginger, Turmeric, Bay leaf, and Coriander, against Escherichia coli, Staphylococcus aureus, Salmonella Typhi, and Pseudomonas aeruginosa. The antibacterial activity of different spices was calculated by the agar well cup plate method. The concentrations, 75%, 50%, and 25%, were made for all the test spices by diluting the concentrated extract with appropriate volumes of sterile distilled water. Ginger and turmeric are effective against all four pathogens. Ginger reported maximum activity against Escherichia coli with 14.5 zones of inhibition at 100% concentration. Bay leaf showed comparatively good antibacterial activity at higher concentrations and possessed maximum antibacterial activity against Salmonella Typhi with 15.4 zones of inhibition at 100% concentration. Coriander unveils comparatively good antibacterial activity against Escherichia coli and Staphylococcus Aureus at higher concentrations. It exhibits maximum antibacterial activity against Pseudomonas aeruginosa with an 18.7 mm zone of inhibition at 100% concentration. Coriander didn’t reveal activity against Salmonella Typhi at any concentration. The results of the present study are...
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Posted by admin on Feb 28, 2022 in |
Aceclofenac is a non-steroidal anti-inflammatory drug indicated for symptomatic treatment of pain and inflammation. Aceclofenac is a BCS Class II drug with low solubility and high permeability, thus solid dispersion is one of the promising techniques used for enhancement of solubility and dissolution rate. The intent of the present study was to formulate and evaluate aceclofenac matrix tablets by Wet Granulation technique, by partial in-corporation of the solid dispersed product as loading dose and pure drug as maintenance dose; so that the initial loading dose will produce an immediate therapeutic effect and the pure drug will lead to maintaining the concentration within the therapeutic index. Solid dispersions of aceclofenac were prepared by solvent evaporation method by using two different carriers klucel Hydroxy Propyl Cellulose–EXF and Hydroxyl Propyl Methyl Cellulose K-100 M CR by varying the drug and polymer ratios. Aceclofenac matrix tablets were formulated by wet granulation technique and all the formulations were evaluated for pre-compression and post-compression parameters. The granules showed good flow properties. All the formulated tablets...
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Posted by admin on Feb 28, 2022 in |
Bell’s palsy is an idiopathic, unilateral, acute weakness of the face in a pattern consistent with peripheral facial nerve dysfunction and may be partial or complete, occurring with equal frequency on either side of the face. The incidence is about 20 in 100,000 people a year, with about 1 in 60-lifetime risks. Bell’s palsy has a peak incidence between the ages of 15 and 40 years. Viral infections are commonly associated with facial nerve pathology, which leads to peripheral facial paralysis. A potential cause of peripheral facial paralysis might be COVID-19 and neurological symptoms could be the first and only manifestation of the disease. Possible mechanisms related to nerve damage in idiopathic facial nerve paralysis include ischemia of vasa nervorum and demyelination induced by an inflammatory process. Direct viral damage or an autoimmune reaction toward the nerve-producing inflammation would be alternative or contributing mechanisms to dysfunction. Acyclovir (aciclovir) is a nucleoside analogue antiviral drug active against some of the herpes virus groups of DNA viruses and RNA viruses. The...
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