Posted by admin on Dec 31, 2022 in |
The present research aims to synthesize novel 5, 6-dihydro-3-(substituted phenyl) [1, 2, 4] triazolo (3, 4-b) [1, 3, 4] thiadiazine-7-one derivatives and their physiochemical evaluation for antibacterial and antioxidant activities. Novel synthetic schemes were designed to synthesize compounds (1-6), which were then characterized using physicochemical and spectroscopic methods (IR, 1HNMR). In-vitro antimicrobial activity was performed against Gram-positive (Staphylococcus aureus, Bacillius subtilis), Gram-negative (Escherichia coli) bacterial strains and fungal strains (Aspergilus niger, Candida albicans) of all synthesized compounds. In addition, Minimum inhibition concentration (MIC) and Minimum fungicidal concentration (MFC) values were determined using tube dilution methods using standards such as norfloxacin and fluconazole. The free radical scavenging activity was also assessed using the DPPH assay, with vitamin C serving as a reference molecule. Compound 3 was discovered to have the best antibacterial activity (IC50– 80.27) of all the compounds, while the rest compounds showed modest scavenging...
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Posted by admin on Dec 31, 2022 in |
BioMarin Pharmaceutical is developing Vosoritide, a modified recombinant human C-type natriuretic peptide (CNP) analogue for the treatment of achondroplasia. A gain-of-function mutation in the fibroblast growth factor receptor 3 gene (FGFR3), a negative regulator of bone growth, causes achondroplasia. Vosoritide works to restore chondrogenesis by attaching to the natriuretic peptide receptor B (NPR-B), which inhibits the hyperactive FGFR3 gene’s downstream signalling pathways. In August 2021, the European Union approved Vosoritide for the treatment of achondroplasia in children under the age of two whose epiphyses are not closed; the diagnosis of achondroplasia should be validated by genetic testing. In the United States, the medication is also undergoing regulatory assessment for the treatment of achondroplasia and clinical trials. Several countries are in the process of developing. The milestones in the development of vosoritide that led to this first approval for achondroplasia in children under the age of two whose epiphyses are not closed are summarized in this article. Vosoritide is a medication that was designed to treat achondroplasia and has shown...
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Posted by admin on Dec 31, 2022 in |
Adhesion of endodontic sealers to Dentine and Gutta percha (GP) provides an understanding of the interactions between the root canal wall and the core material. The interaction of Bioceramic sealers has been studied previously but the wetability to Gutta percha has rarely been evaluated. Hence, the present in-vitro study was designed to evaluate and compare the wetability of five different root canal sealers with GP by measuring the sealers’ static contact angles (SCA). Hundred flat and smooth samples of GP were prepared and randomly divided into five groups (n=20) based on the sealer used to wet the surface of GP. Group 1: AH Plus, Group 2: Gutta Flow, Group3: Calpex, Group 4: MTA Fill apex, Group 5: Endosequence BC Sealer. Controlled (0.1 ml) volume droplets of each sealer were placed onto the flat GP surfaces using a sessile drop method, and static contact angles were measured using a KRUSS Goniometer. The mean values of all the samples were calculated, and the data were statistically analyzed using One-way ANOVA and...
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Posted by admin on Dec 31, 2022 in |
This study aimed to analyze antioxidant activity and quantify alpha-asarone by using High-performance thin-layer chromatography (HPTLC). Alpha-asarone is a secondary metabolite in hydroalcoholic (1:1) rhizome extracts of Acorus calamus L. (AC), a medicinal plant being used around the globe traditionally. Sweet flag, AC is a member of the Acoraceae family, and is commonly used unaided as well as in conjunction with other plants in Chinese traditional medicine and the Indian system of medicine. It has sparked a lot of curiosity and has proven to be useful in managing many diseases. Acorus calamus and/or its bioactive phytochemical α-asarone, is a well-known medication in a traditional system with various properties. Antioxidants are extremely essential compounds that can defend the body from harm caused by oxidative stress generated by free radicals. HPTLC is a viable option for expanding chromatographic fingerprints in order to identify medicinal plant’s key active components. At 100µg/mL, the antioxidant activity of AC demonstrated 52.59 percent and increasing to 56 percent at 500µg/mL. HPTLC fingerprinting profiles revealed the presence...
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Posted by admin on Dec 31, 2022 in |
Estimation of creatinine clearance (Clcr) has long been a problem in critically ill patients with unstable kidney function. The commonly used methods like the Cockcroft-Gault method require a stable kidney function. A reliable and more accurate tool is needed to estimate Clcrto to guide drug dosage adjustment. The study aimed to compare the eight methods to estimate Clcr with measured Clcr. In addition, the study also aimed to determine the agreement between estimated Clcr with measured Clcr. This was a multicentre, prospective, observational study. Three intensive care units in Malaysia tertiary public hospitals. Two serum creatinine samples over 24 hours apart and simultaneously 24 hours urine collection. A total of 43 patients were recruited. During the early phase of unstable kidney functions (regardless of acute deteriorating or acute improving), only the modified Cockcroft-Gault method showed a non-significant different with the measured Clcr (p = 0.741). A sub-set analysis on 23 patients with acute deteriorating kidney functions was performed. Only the modified Cockcroft-Gault revealed a non-significant different with the measured...
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