Posted by admin on May 31, 2023 in |
Oxidative stress and cholinergic deficit are major culprits of cognitive impairment. Intake of green leafy vegetables – rich in antioxidant phytoconstituents- strongly correlates with memory-preserving capacity. Our earlier work showed that hydromethanol (HM) extract of Chenopodium album (a common leafy vegetable) had strong acetylcholinesterase inhibitory and antioxidant activity in-vitro. The objective of the present study was to isolate the bioactive fraction/constituent from HM extract of C. album and substantiate its memory-enhancing capacity with in-vitro and in-vivo evidence. The HM extract was fractionated by solvent portioning and column chromatography and the antioxidant and acetylcholinesterase inhibition by fractions and sub-fractions thus obtained were assessed by DPPH and Ellman assays, respectively. The fraction F6 showed best potential in-vitro. Subsequently, the anti-amnesic capacity of HM extract and F6 was assessed using a scopolamine-induced memory impairment mouse model. Morris Water Maze test was used to determine memory. Biochemical parameters and histopathological studies of brain were also evaluated to elucidate anti-amnesic mechanism. In-vivo studies confirmed that F6 protected against scopolamine-induced memory impairment and significantly reduced...
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Posted by admin on May 31, 2023 in |
Using plant extracts to synthesize metal nanoparticles is one of the easiest, most convenient, cost-effective, and ecologically acceptable ways of avoiding hazardous chemicals. As a result, many environmentally acceptable methods for the fast production of silver nanoparticles utilizing aqueous extracts of plant components such as leaves, bark, and roots have been described in recent years. This study highlights and expands on recent discoveries in the green production of silver nanoparticles (AgNPs) utilizing various plant extracts and their prospective uses as antimicrobial agents. A systematic in-depth discussion on the possible influence of phytochemicals and their concentrations in plant extracts, extraction solvent, and extraction temperature, as well as reaction temperature, pH, reaction time, and precursor concentration. Extensive details of the probable mechanism of AgNPs’ interaction with microbe cell walls, which leads to cell death and strong antibacterial activity, have also been developed. Furthermore, even though chemical techniques for shape-controlled synthesis are well-known, regulating the shape of biosynthesized AgNPs has numerous beneficial impacts on its...
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Posted by admin on May 31, 2023 in |
Two simple and sensitive evulsions spectrophotometric methods have been described for the assay of Cycloserine either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion – pair complexes of the drug with bromophenol blue (BPB) and bromo cresol green (BCG) in acidic medium. For two methods, the extracted complexes showed absorbance maxima at 408 and 419.5nm. Beer’s law is obeyed in the concentration ranges 2.0-20 and 2.5-25μg/mL with BPB and BCG respectively. The effects of concentration of dye, pH and interference of recipients have been studied and optimized. The limit of detection and quantification has been determined for two methods. These two are very accurate methods of physical and instrumental mode of experiments. Two methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery...
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Posted by admin on May 31, 2023 in |
Central and eastern Brazil is home to the cactus species Cereus jamacaru, also called mandacaru or cardeiro. It frequently reaches a height of 6 metres. For animal feed, a kind without thorns is utilized. The most typical type is quite thorny; however, if the thorns are The native Brazilian cactus Cereus jamacaru D.C., also known as “mandacaru,” grows naturally in the Caatinga biome and is characterized as a source of food and medicines used to cure kidney inflammation, urinary infection, and rheumatism. However, the antioxidant activity and phenolic chemicals have not yet been assessed. The current study’s phytochemical examination of the ethanolic extract to assess the phytochemical, antioxidant, and anti-diabetic effects of C. jamacaru root extracts showed the Presence of terpenoids and flavonoids, saponins, alkaloids, steroids, coumarin and carbohydrates. This study has demonstrated that C. jamacaru root extract contains primary and secondary metabolites that can be pharmacologically useful and possess some antidiabetic and antioxidant properties. It was also discovered that C. jamacaru root ethanol extract possessed antioxidant and antidiabetic...
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Posted by admin on May 31, 2023 in |
Coronavirus disease 2019 (COVID-19) pandemic has emerged as a major healthcare problem and has posed a great challenge to our existing healthcare facilities. Treatment of COVID-19 has been primarily based on repurposed drugs, drugs approved under emergency authorization or other options which are not thoroughly evaluated in patients with COVID-19, hence, lacking an adequate data on safety and efficacy. Public awareness and education regarding the potential benefits and safety concerns of the available therapeutic/prophylactic options are crucial to avoid ethical and medicolegal issues. Fundraising and global partnership are required to raise the research on potential older drugs likely to be repurposed, along with novel treatment options for COVID-19. A global effort is required to ensure the availability, distribution, and safer administration of COVID-19 vaccines. A rational and ethical approach is required to manage the patients with COVID-19. Equitable access to COVID-19 vaccines should be a priority to end this pandemic. In this review, we have focused on concerns regarding ethics and rational medicine use in view of the available...
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