Posted by admin on May 31, 2023 in |
Pyrazole and its derivatives are pharmacologically active and are being used in various disorders and diseases with a few side effects. The wide distribution of pyrazole compounds in nature with fascinating pharmacological action is key in the application of this development of newer derivatives. The main structure has a great variety of compounds that have essential pharmaceutical and agrochemical activities in addition to biological activity like anti-inflammatory, antifungal, antitumor, antiviral, antibacterial, anti-tubercular, and antiphrastic. Protection against various substances that causes oxidative stress in the body is needed in the modernizing world in this direction intermediate obtained from the Vilsmeier Haack reaction (4) was treated with aromatic amines to obtain N-[3-(naphthalen-1-yl)-1-phenyl-1H-pyrazol-4-yl] methylene) derivatives (5a-5l). The in-vitro antioxidant activity of newly synthesized compounds was determined by the Nitric oxide scavenging...
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Posted by admin on May 31, 2023 in |
A Quality design approach to method development involves method goal identification, method scouting and evaluation, method selection, and risk assessment. The present study describes the risk-based HPLC method development and validation of Formoterol fumarate and Aclidinium bromide in a pharmaceutical dosage form. The chromatographic conditions were optimized with the Design Expert software 11.0 version i.e., Kromasil C18 (250×4.6 mm, 5 µm) Mobile phase used was 0.01N Kh2po4 (pH :3.0) (52.4%): Acetonitrile (47.6%), flow rate was found to be 1.0ml/min with retention times of formoterol 2.456 min and Aclidinium 3.573min. The developed method was found to be linear in the range of Formoterol 1.5-9μg/ml, aclidinium 50-300μg/ml with a correlation coefficient of 0.999 for both drugs. The % RSD of intraday and inter-day precision for Formoterol 0.3 & 1.0, Aclidinium 0.7 & 0.4. The robustness values were less than 2%.The assay was found to be 99.91%&99.99% for formoterol and aclidinium bromide. The method validation parameters were in the prescribed limit as per ICH guidelines. Stress studies reveal that both drugs were...
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Posted by admin on May 31, 2023 in |
Antimicrobial resistance is a threat to public health. This study investigated the occurrence of ESBL and colistin resistance among Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa recovered from clinical samples (n=500) in a tertiary teaching hospital in Abakaliki, Nigeria. The samples were bacteriologically analyzed using selective culture media, biochemical tests, and PCR. All isolates were screened for ESBL and colistin resistance genes using both PCR and disk diffusion methods that included standard antibiotic disks for susceptibility studies. E. coli (n=173), K. pneumoniae (n=120) and P. aeruginosa (n=62) were recovered from the clinical samples investigated. The most prevalent ESBL genes detected is blaCTX-M found in 3 isolates of P. aeruginosa, 4 isolates of E. coli and 2 isolates of K. pneumoniae. The colistin resistance gene, mcr-3 was detected in 4 strains of E. coli for the first time in Southeast Nigeria. Both mcr-1 and mcr-3 was not detected in the K. pneumoniae strains. Only the mcr-2 colistin resistance genes were detected in K. pneumoniae strains. There was no detection of...
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Posted by admin on May 31, 2023 in |
Atherosclerosis is a chronic inflammatory disease that is marked by atheroma and patchy intimal plaques in arteries that cause thickening and hardening of arteries. It is reported that “Origanum majorana L.” possess antiulcerogenic, antiproliferative, antioxidant, and anticarcinogenic activities, and it is an important remedy against thromboembolic disease. The present study was designed for the evaluation of the anti-atherosclerotic activity of hydrodistilled volatile oil (OMO) of Origanum majorana L. in high-fat diet-induced atherosclerotic rats. The in vitro studies found that OMO possesses fibrinolytic, thrombolytic, and antiplatelet activity. These activities can play an effective role in the anti-atherosclerotic activity of OMO. Atherosclerosis was induced using a high-fat diet model in atherosclerotic activity of OMO. Atherosclerosis was induced using a high-fat diet model in histopathological examination of the aorta was determined at the end of the study. OMO significantly showed that the levels of total cholesterol (TC), triglyceride (TG), low-density lipid (LDL), and very low-density lipids (VLDL) levels were also decreased, there is a decrease in the level of liver function enzymes...
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Posted by admin on May 31, 2023 in |
Background: To enhance patient compatibility Safoof e Muhazzil (powder) was compressed into tablets. Objectives: The present study was aimed at comparing the two dosage forms for any variations in the efficacy of Safoof e Muhazzil. Method: This triple-armed RCT was carried out on 90 patients after obtaining ethical clearance and written and informed consent. The study period was six weeks. Improvement was measured in terms of change in weight, BMI, waist hip ratio (WHR) and waist circumference (WC). 5g of powdered Safoof e Muhazzil (SMC) was given twice daily with water, whereas, 5 tablets of Safoof e Muhazzil (SMT) were given twice daily with water. The third group was given atarvostatin 10 mg once daily. Result: The study recorded a 20.03 % dropout. Most of the patients were non-alcoholic, non-smoker, non-vegetarian males above 45 years and had BMI between 25-30 kg/m2. SMC and SMT gave similar results on all parameters of weight management with p<0.001. This can be attributed to the anti-oxidant and lipolytic properties of Safoof e Muhazzil...
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