Posted by admin on Apr 30, 2023 in |
This study describes the implementation of a Quality by Design approach to develop and validate the RP-HPLC for amlodipine and valsartan. The reaction surface method optimizes by adopting the three-level Box Behnken design. The three factors selected are methanol and water concentrations (mobile phase), flow rate, and wavelength. The developed chromatographic method was validated against the ICH Q2(R1) guidelines for linearity, range, precision, LOD, and LOQ. The maximum absorbance of amlodipine and valsartan (λ max) was found to be 245 nm. The optimized method consists of mobile phase Methanol: Water (pH 3.0) (80:20), and flow rate 0.9 ml/min, which was optimized by using design expert software. Linearity of the developed method was established over the concentration range of 1 10 μg/ml for Amlodipine and 30 – 200 μg/ml with correlation coefficients (r2) of 0.997 and 0.9993, respectively. The percent RSD for accuracy and precision of the method was found to be less than 2%. The limit of detection (LOD) was 0.08 µg/ml and 0.89 µg/ml for Amlodipine and Valsartan,...
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Posted by admin on Apr 30, 2023 in |
This research aimed to develop an inclusion complex of resveratrol (RSV)/hydroxypropyl-β-cyclodextrin (HP-β-CD) using a quality-by-design (QbD) approach to improve the solubility and stability of RSV and formulate it into a fast-dissolving sublingual film (FDSF). The RSV/HP-β-CD inclusion complex was prepared by the kneading method using a Box–Behnken Design to assess the effect of the molar ratio of RSV and HP-β-CD, reaction time, and drying time as independent variables on the % entrapment efficiency and % yield. FTIR, DSC, Powder XRD, NMR, and SEM were performed to evaluate the formed complexes. The RSV/HP-β-CD complexation was successful in enhancing RSV solubility and stability. Furthermore, the inclusion complex was incorporated into a fast-dissolving sublingual film. The prepared films were evaluated for a folding endurance test, tensile strength, disintegration time, drug content, thickness, and SEM. The sublingual route of administration was found to be beneficial for films, as it provided 80% permeation of RSV in 60 minutes and no damage to the epithelial tissues was observed in the histopathological study performed on the...
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Posted by admin on Apr 30, 2023 in |
Pancreatic cancer is an intractable and rare malignancy ranked 12th in incidence and 7th in mortality. It is the leading cause of cancer deaths in developed countries and is rising in developing countries like India. According to GLOBOCAN 2020 statistics, pancreatic cancer has ranked as the 11th most common malignancy in the world and accounts for 495773 new cases and caused 466003 deaths in 2020. Tumor hypoxia is an important pathological condition that influences several signaling cascades in malignant cells, eventually leading to therapy resistance. Several natural bioflavonoids hold promise as anti-cancer agents. However, the effect of Quercetin on hypoxic pancreatic cancer cells is unknown. We analyze the effect of Quercetin on expression levels of metastatic markers HIF1α and E-cadherin under hypoxic conditions. Quercetin treatment was significantly correlated with reduced HIF1α expression levels and elevated E-cadherin levels. Our results suggest that Quercetin may alter E-cadherin levels by regulating HIF1α expression. Quercetin may inhibit pancreatic cancer cell metastasis by regulating metastasis genes in a hypoxic microenvironment and has pharmacological potential...
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Posted by admin on Apr 30, 2023 in |
Novel drug delivery approach by using natural polymers is a newer area of formulation development. The oral drug delivery in the buccal cavity to treat the dental infections has some limitations such as slow onset of actions and is also affected by the first pass metabolism. In order to overcome these challenges plant derived mucoadhesive gum from the fruit pulp of Ziziphus mauritiana L.(ZM) is used in the preparation of Denticap and evaluated in vitro for to analysis its sustained release action. Antibacterial drug Amoxycillin Trihydrate has been taken as the model drug. The denticap was formulated using Carbopol 934P, Z Mgum, Ethyl cellulose and ethanol by solvent evaporation technique. The physicochemical parameters such as swelling index, surface pH, tooth adhesion test etc were carried out. The swelling % ranges from 40-55%. The tooth adhesive strength and pH was 45g and 6.7 respectively. The cumulative drug release from the ZM denticap was found to be 67% at 30 hr. The drug release kinetic study shows that formulation follows diffusion...
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Posted by admin on Apr 30, 2023 in |
Background: Infections occur when viruses, bacteria, or microbes enter the body and multiply. Control of infection is achieved by using antibiotics; hence scrutiny of antibiotic prescribing patterns is an important aspect of quality and standards of clinical practice. Objective: To evaluate and analyse drug utilization and prescription pattern of antibiotics in various in-patient departments of the hospital to ensure safe and effective use. Methods and Material: A Retrospective Observational Study was carried out in 6 inpatient departments of Vivekananda General Hospital, Hubballi over a period of 1 year. A total of 993 prescriptions were collected, and the results were analyzed using MS Excel 2020, and SPSS 16.0. Results: This study reveals that the majority of patients belonging to the age group 41-60 years, received antibiotic therapy. Most patients were prescribed with antibiotic Ceftriaxone, 21.19% (n=402). Majority of the patients received antibiotics for Prophylactic treatment (44.65%). It was found that most of the patients were prescribed only one antibiotic (43.1%) whereas only 2.51% of patients were prescribed more than 4...
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