Posted by admin on Mar 31, 2023 in |
Different-different modification of Benz-oxazole moiety gives various numbers of novel compounds which shows various biological activities. Some new derivatives (G7-G10) containing Benzoxazole moiety were prepared by the use of different methods and reagents. O- Phenylenediamine treated with Chloroacetic acid to form 2(chloro methyl) benzimidazole (compound B) and 4- methyl, 2- amino phenol with methyl alcohol and carbon di sulfide to form 2-thio, 5- methyl, benz-oxazole (compound G1). Further Compound B treated with (compound G1) to form [(benz-imidazole)-2-methyl thio]-5-methyl, benzoxazole (compound G2). Finally compound G2 treated with a substituted aldehyde to form benzoxazole derivatives (G7-G10). Compound spectral analysis was done by Infrared spectroscopy (IR), 1H-Nuclear magnetic resonance (1HNMR) & Liquid chromatography-mass spectroscopy (LCMS). The serial Dilution method (MIC test) was used for anti-bacterial activity, and Alamar blue assay test was used for Anti-tubercular activity. Compounds G8 and G9 showed potent anti-tubercular activity against M. Tuberculosis, and Compounds G7, G8 and G10 were showed good antimicrobial...
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Posted by admin on Mar 31, 2023 in |
Medicinal plants play a vital role in human health as these are nature’s gift to human beings to make disease-free, healthy lives. The various families such as Amaranthaceae, Cucurbitaceae, Euphorbaceae, Fabaceae, Malvaceae, Myrtaceae, Orchidaceae, Piperaceae, Solanaceae etc. comprises several medicinal properties distributed in the tropical and subtropical region of India. The medicinal usage of these families has been reported in the traditional systems of medicine. An exhaustive literature survey was performed on the medicinal plants of the district which revealed that 90 plants belong to different families is reported antiulcer property used by tribal region. Different parts of Fabiaceae family plant extract are widely used by tribal (Gond Madiya Jamat) of Gadchiroli to heal ulcer and relieve stomach pain without precipitating any side effects. The present review was conducted and focuses on the ethnomedical, phytochemical and antiulcer activity of different plant extracts of the Fabiaceae...
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Posted by admin on Mar 31, 2023 in |
Adrenergic receptors (ADRs) are implemented in important pharmacological responses. The β3adrenergic receptors have been proposed as a suitable drug target for treating diabetes and obesity. However, very little information is available regarding the expression profile of β3 receptors. We have designed primers for studying the β3adrenergic receptors in C57BL/6J mice using various bioinformatics tools in the present study. The primers were designed using three different software, primer 3 Plus, NCBI Primer BLAST, and Primer express, and validated using UCSC in-silico PCR and NCBI -Primer-BLAST. The best primer pair was selected based on primer melting temperature, GC% and primer length. The selected primer pair was custom synthesized. The primer annealing temperature was standardised using gradient PCR. Among the three software used for primer designing, primer express was found to be the best, and the primers designed from primer express were found to have the ideal melting temperature between 58-60°C, 50% of GC content, and 20bp length. From gradient PCR, the primer annealing temperature was found to be 58.3°C. The bioinformatics tool is...
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Posted by admin on Mar 31, 2023 in |
Spironolactone belongs to a group of medicine known as aldosterone antagonist used as sparing diuretic, Spironolactone (aldactone) is potassium -aspiring diuretic agent and has half-life of about 16 hours. Spironolactone one diuretics agents which increases renal excretion of water and solutes (mainly sodium salt). It is used mainly in the treatment of refractory edema in patients with congestive heart failure nephrotic syndrome, or hepatic cirrhosis. On its own, spironolactone is only a weak diuretic, but it can be combined with other diuretics. Spironolactone is 7α-acetyle thio-3-oxo-17α-pregn-4-ene-21,17β- carbolactone. Its molecular formula is C24 H32O4S having a molecular weight 416.58 gm/mole. The possible related compound (degradants and process-related impurities) as Spironolactone related compound –A of Spironolactone having chemical names are S-((2’R, 7R, 8R, 9S, 10R, 13S, 14S)-10, 13-Dimethyl-3, 5′-dioxo-1, 2, 3, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16-tetradecahydro-5’H-spiro[cyclopenta[a]phenanthrene-17,2′-furan]-7-yl)...
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Posted by admin on Mar 31, 2023 in |
Marine environment is an enormous pool of biodiversity resources that cover approximately 70% surface of the earth. Marine microorganisms have unique properties since they have to adapt to extreme marine environmental conditions in deep-sea water. The present study aims to isolate, screen and identify bacterial strains from marine sediment samples that produce an enzyme pectinase with clinical and industrial applications. Marine sediment samples were collected and cultured on zobell marine agar medium. After incubation, five bacterial strains were screened for pectinase activity. Two isolates showed pectinase activity on Yeast Extract Pectin (YEP) medium. Morphological and biochemical characteristics of pectinase-producing organisms were done. Pectinase-producing bacterial strain (PE S7C) was selected based on their pectinase test. The selected strain PES7C was further sequenced for identification. The phylogenetic characterization and 16S rRNA of the strain PE S7C revealed that bacterial cultures belong to Bacillus cereus. The morphological studies indicated that the isolate was Gram +ve, rod-shaped and motile organism. The present study concludes that marine bacteria can be a source of pectinase with...
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