Posted by admin on Mar 31, 2023 in |
Neurodegenerative diseases (NDs) that are characterized by progressive loss of functioning and structure of neurons are of great burden to the individuals and the society. With several hypotheses, the existent cause still remains a mystery for various NDs in healthcare. Protein degradation, inflammation, oxidative stress, environmental factor, mitochondrial defects, abnormal protein accumulation in neuron and familial history are amongst the commonly studied environmental causative factors for NDs. These disorders are a significant cause of disability and mortality, and consequently increasing life spans is one of the key challenges for medical research. The excessive treatment cost has led the world to move to alternative therapy with lower cost and minimal side effects compared to conventional treatments. Medicinal plants have been used for treating various diseases since ancient times in India. Medicinal plants used in Unani System of Medicine cover most of the NDs. It has defined a huge number of plants with multiple therapeutic benefits. In this review, the role of important medicinal plants, viz., Prunella vulgaris L., Melissa officinalis...
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Posted by admin on Mar 31, 2023 in |
The present study investigated the effect of intake of aspartame in diabetic rats, a widely used artificial sweetener (aspartame), on antioxidant defence status in the rat brain. Adult male Wistar rats weighed 180 ± 20 gm were randomly divided into 4 groups as follows: The first group was control; the second group was given aspartame (ASP) at a dose of 50 mg/kg body weight; the third group was Diabetes (D) (Streptozotocin (STZ) 45 mg/kg body weight); and fourth group was Diabetes rats (STZ 45 mg/kg body weight) administered with ASP (D+ASP) (50 mg/kg body weight). Induction of Diabetes (STZ) through intraperitoneal injection at a single dose. ASP is administered with gavage for up to 30 days. Superoxide dismutase, catalase and glutathione reductase are significantly reduced. In contrast, in lipid peroxidation, a significant increase was observed in the various brain regions of the experimental group rats compared with the control group rats. The results indicate that consumption of aspartame leads to an imbalance in the normal and diabetic rats’ antioxidant/pro-oxidant...
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Posted by admin on Feb 28, 2023 in |
Glutathione peroxidase gene expression profiling has been determined using budding yeast, S. cerevisiae, as a model organism mainly used for extensively reliable microbial studies of various cellular processes evolved in evolutionarily distant species. Roots of five medicinal plants were used: Withania somnifera, Terminalia arjuna, Ranunculus sceleratus, Bacopa monnieri and Acalypha indica. The maceration method exhibited the extraction process, followed by S. cerevisiae culturing at 600nm. Classic Trizol method for RNA extraction of yeast cells. Further, evaluation of the quantity and quality of the isolated RNA before the CDNA synthesis, respectively. Polymerase Chain Reaction was performed using specific conditions, having 60OC as GPx primers annealing temperature. Real-Time Polymerase Chain Reaction of samples was performed where Beta-actin primers as a housekeeping gene. High purity of RNA samples was yielded to be in the range of 1.8-2.0. The size of cDNA PCR products was found to be 118bp. The RT-qPCR results showed high over-expression, i.e., fold change unit of GPx gene in all sample extracts compared to the control yeast. Ethanol and...
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Posted by admin on Feb 28, 2023 in |
Nanoparticles are small particles ranging from 1 to 100 nanometers. The biological production of nanoparticles is cheaper than chemical production. Besides being cheaper, it is eco-friendly as well. This biological procedure is a bottom-up procedure where the extracts of natural products replace the chemical reducing agents. These reducing and stabilizing agents are found in plants and can be used to synthesize nanoparticles. Plants also have growth terminating and capping properties as well. This paper aims to give brief information on different plant sources and synthesis of nanoparticles. The infection caused by multi-drug-resistant bacteria increases the mortality rate. So, the effect of multi-drug-resistant bacteria is gaining much more importance. The nanoparticles synthesized by green sources are non-toxic to the environment and humans. This review focuses on basic knowledge of nanoparticles, their production by green sources, the application against multi-drug-resistant bacteria, and advantages and disadvantages of...
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Posted by admin on Feb 28, 2023 in |
Benzodiazepines are psychoactive drugs used to treat anxiety, epilepsy, convulsions, and psychiatric and sleeping disorders. These medicinal compounds possess considerable importance in forensic toxicology due to their tranquilizing, hypnotic, and anticonvulsant properties. Thus, identifying and separating these compounds found significance in pharmaceuticals. The high-performance liquid chromatography (HPLC) technique is employed to separate benzodiazepines. The present research article aims to provide a more straightforward and efficient method for separating benzodiazepines. The thin layer chromatography (TLC) technique follows to separate benzodiazepines containing silica gel 60 F254 as a stationary phase. The seven different mobile phases for the separation of benzodiazepines are employed. The combination of Acetone: Toluene: Ethanol: Ammonia (45:45:7:3) is the best solvent system for separating benzodiazepines among the seven chosen solvent...
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