Posted by admin on Jan 31, 2024 in |
The pyrimidine scaffold found in many bioactive drugs; some commercially available drugs containing at least one pyrimidine ring in their structure. The literature survey reveals that replacement of 1H-pyrazole of pyrazolo [3,4-d] pyrimidine ring system by other bioactive moieties drastically alters its pharmacological properties. Prompted by the varied biological activities of pyrazolopyrimidine derivatives, we envisioned our approach towards the synthesis and antimicrobial screening of a novel series of pyrazolo [3,4-d] pyrimidine derivatives. Novel functionalized pyrimidine derivatives and their derived bicyclic pyrazolo thiazolo pyrimidine compounds were synthesized by cyclocondensation of 3-cyano-2- methylthio-4-oxo-4H-substituted thiazolo [1,2-a] pyrimidines with hydrazine hydrate gave novel series of pyrimidine derivatives in one step, and the chemical structures of the compounds was confirmed by IR and 1 H NMR spectral data. All the compounds of the series have been screened for their antibacterial and antifungal activity studies. The result revealed that all compounds showed significant antimicrobial activity. The current research is being directed towards the synthesis and improvement of biological activity of the pyrazolopyrimidine...
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Posted by admin on Jan 31, 2024 in |
Introduction: Urinary tract infection is the most frequent infection reported worldwide till now. To better understanding regarding epidemiology and emerging antimicrobial resistance among bacterial uropathogens, we studied the demographic distribution and antibiotic susceptibility pattern of microorganisms causing UTI in our local hospital-based study. Materials and Methods: We analysed the retrospective data of laboratory confirmed UTIs, from January 2017 to December 2021. Causative agents of UTI, along with their demographic profile, distribution and their antibiogram was determined by using conventional methods. On the basis of that, 5-year multiple antibiotic resistance index was calculated to determine the resistance pattern of isolated bacterial uropathogens. Results: During study period, total 9426 samples were received. Out of which, 1133 (12.01%) showed significant bacteriuria with monomicrobial growth. With 64.17% isolation from female patients with predominance age group 16-60 years. From 1133 positive isolates, gram negative and gram positive isolates were reported in 298 (26.30%) and 835 (73.69%) isolates respectively, with predominance of E. coli (52.25%) followed by Enterococcus spp. (18.36%) Klebsiella spp. (8.21%). Multiple antibiotic...
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Posted by admin on Jan 31, 2024 in |
Human gut serves as a home to over 100-1000 microbial species, which primarily modulate the host internal environment and thereby, play a major role in host health. Humans are a unique reservoir of heterogeneous and vivacious group of microbes, which together forms the human-microbiome superorganism. Probiotic bacteria are beneficial to human health by improving the gutmicro biota balance and the defenses against pathogens. Additional health benefits attributed to probiotics are the stimulation of the immune system, blood cholesterol reduction, vitamin synthesis etc. Chocolate is mostly a unique food product for all age groups. The addition of lactobacillus to these chocolates thus creates a tastier option for transferring these bacteria to the stomach for the betterment of the microbial environment of the gut. Thus, Lactobacillus and Bifido bacterium were collected from five different samples and introduced into the milk chocolate for the preparation of probiotic milk chocolate. Both the organism was isolated and identified. Their physiochemical properties, sensory attributes and probiotic viability in milk chocolates were assessed. Thus, the study...
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Posted by admin on Jan 31, 2024 in |
Ajụ mbaise polyherbal extract, widely consumed for medicinal and nutritional purposes is composed of stems, leaves, roots and barks of different plant species wrapped together in various proportions. The current study was carried out to determine the effects of orally administered ethanolic Ajụ mbaise polyherbal extract on lipid profile and blood glucose levels of alloxan-induced diabetic rats. Twenty male rats of Wistar strain were randomly assigned into four groups of five rats each, following a 7-day acclimatization. Group A served as normal control, Group B as diabetic control, while Groups C and D were diabetic and orally administered with 200 and 400 mg/kg body weight of Ajụ mbaise polyherbal extract respectively. Extract administration lasted for fourteen days. After which, blood glucose level was checked on overnight fasted rats. Thereafter, animals were anesthetized and blood samples obtained via cardiac puncture were used for biochemical estimation of the lipid profile. Two weeks post extract administration, the blood glucose level was significantly increased (P < 0.05) in the diabetic non-treated group, while...
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Posted by admin on Jan 31, 2024 in |
A precise, accurate, robust method was developed for quantitative estimation of related impurities of Metformin HCL and Vildagliptin from combination tablet dosage form. The mixture of ammonium dihydrogen orthophosphate and octane sulfonic acid sodium salt buffer at pH 4 was used as mobile phase A and mixture of methanol and buffer in the ratio of95:5 used as a mobile phase B. The mobile phase was pumped at 0.8 ml/minutes flow rate through BDS Hypersil C8, 250 x 4.6 mm, 5µ, HPLC column operated at 35°C. All the solutions were injected at 10µl injection volume, and the chromatograms were monitored at 210 nm based on optimum response of impurities and analytes. The % recovery of Metformin HCL, was found 90.4, 92.3, 99.4 and 99.2%, while for Vildagliptin was 90.5, 94.5, 99.6 and 98.3 % from LOQ, 50,100 and 150 % levels respectively. The method was found linear from LOQ to 150% with the correlation coefficients (r2) were 0.998 for Metformin HCL and Vildagliptin. No interference observed from diluent and placebo...
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