Posted by admin on Oct 31, 2017 in |
Over time, ethnomedicinal plants have been praised hugely for the presence of diverse pharmacologically active phytochemicals and have been used traditionally in primary medication to cure various human diseases or disorders. In the present study, different phytochemical constituents were analysed and free radical scavenging activities of Dregea volubilis stem bark (DvSB) extracts were assayed. Different in vitro scavenging tests such as DPPH· scavenging assay, O2·– scavenging assay, OH· scavenging assay and H2O2 scavenging assay were employed to estimate the free radical scavenging potentiality. Results revealed that methanolic extract had superior scavenging activity over the hot water extract. Qualitative analysis of phytochemicals showed the presence of reducing sugars, phenolic compounds, flavonoids, resins, glycosides, cardiac glycosides and steroids; and quantitative phytochemical analysis for polyphenols, flavonoids, flavonols, proanthocyanidins and alkaloids were made by standard procedures. Hence the study reveals that DvSB has significant scavenging properties, thus validating the ethnic usage for treating stress-related complications and restoration of good...
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Posted by admin on Oct 31, 2017 in |
The objective of the present study is to identification of the oxidative degradation impurity in the Granisetron hydrochloride active ingredient and also the novel method developed based on the forced degradation studies performed on Granisetron Hydrochloride to well resolve the impurities from the API. Forced degradation studies were conducted as per ICH technical requirements prescribed stress conditions using acid, base, oxidative, thermal stress, and photolytic degradation to show the stability indicating power of the method. Significant degradation was observed during basic hydrolysis and in oxidative studies and no degradation was observed in other stress conditions. Characterization of oxidative degradation impurity was conceded with a combination of analytical and preparative LC isolation followed by LC-MS/MS, IR and 1H and 13C NMR and 2D NMR spectral data. The HPLC chromatographic conditions were chosen to achieve satisfactory resolution between the impurities and API with shortest analysis time. Granisetron hydrochloride and its impurities were separated on Xbridge phenyl column (150mm×4.6 mm, 3.5 microns particle size). The mobile phase A composed of 10mM of...
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Posted by admin on Oct 31, 2017 in |
Background: Lenalidomide plus Dexamethasone (Len-Dex) and VAD (Vincristine, Doxorubicin and Dexamethasone) regimen are the two common drug therapies involved in the treatment of Multiple myeloma. These two groups of drugs act by different mechanisms and their safety profile also varies. Objectives: To compare the safety of Len-Dex versus VAD regimen based on World Health Organization toxicity criteria by grade as well as the performance status of the patients of both the regimen by using Karnofsky performance status scale definitions rating. Materials and Methods: Eighty patients (forty in each arm) of newly diagnosed cases of multiple myeloma, who were willing to give the informed consent, were included in the study. Their baseline investigations and follow up investigations were collected at regular intervals, based on these values, the adverse effect profile as well as the performance status were evaluated and the results were compared and analyzed. Results: In Len-Dex regimen, constipation, leucopenia, thrombocytopenia, slow wound healing, sedation, renal toxicity and hepatotoxicity were high. VAD regimen produce higher incidence of nausea, vomiting,...
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Posted by admin on Oct 31, 2017 in |
Candidemia is associated with a high mortality. The most common cause of candidemia is Candida albicans, though infections by non-albicans Candida are being increasingly reported. The present study was conducted to determine the incidence of candidemia, the risk factors and antifungal susceptibility in intensive care unit (ICU). This study was conducted from January 2012 to June 2013 and prospectively included patients admitted in ICU for >48 hours. The blood culture isolates were identified as per standard mycological procedures and DNA (26S rDNA) sequencing. Antifungal susceptibility test was carried out by broth micro-dilution method in accordance with CLSI standards. A total of 48 isolates were identified in 1890 ICU admissions. Majority of the isolates were identified as non-albicans Candida i.e. 38 out of 48 isolates which is 79.2% while only 10 isolates were that of Candida albicans i.e. 20.8%. Among the non albicans species, Candida tropicalis (42.1%) was the predominant one followed by Candida rugosa (26.3%), Candida parapsilosis (15.8%) while Candida pelliculosa, Candida glabrata, Candida lusitaniae and Kodamea ohmeri were...
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Posted by admin on Oct 31, 2017 in |
Eutyphoeus gammiei (Beddard) is a large native earthworm which is commonly used as fish bait and feed of chicken in Tripura. Eutyphoeus tissue comprising of both body wall and gut wall contains 63.98% protein, 15.79% carbohydrate, 7.78% fat, 1.90% crude fiber and total ash 10.55%. The result showed that essential amino acid of earthworm tissue was dominated by lysine (1.45 g /100 g), methionine (1.56 g /100 g), valine (2.78 g /100 g) and leucine (5.52 g /100 g). The non essential amino acid of earthworm was dominated by glutamic acid (1.37 g /100 g) and ornithine (3.68 g /100 g). Interestingly, the proportion of unsaturated fatty acids was relatively high (poly unsaturated fatty acids 26%, mono unsaturated fatty acids 27.4%). In poly unsaturated fatty acids, arachidonic acid (20: 4 ω -6) and linoleic acid (18: 2 ω -6) represented 36.39% and 29.50% respectively. Importance of essential amino acids and essential fatty acids in earthworm tissue for animal nutrition has been given in the present paper. Nutritionally important several...
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