Posted by admin on Sep 30, 2017 in |
Association of memory impairment (MI) with epilepsy, majorly in temporal lobe epilepsy (TLE) is considerably a tricky condition where existing treatment options involving the administration of anti-epileptic drugs (AED’s) tend to worsen the stated incidence. This study evaluates the aggravating effect of valproate (VAL) at normal and reduced doses on MI associated with TLE and additionally assesses the protective effect of levetiracetam (LEV) on this undesirable link. Albino mice of either sex (n = 36) were employed for the study period of 64 days where seizures were induced by intraperitoneal administration of pilocarpine followed by evaluation of anti-epileptic activity by technique of Racine scores. Assessment of errors, a factor for degree of MI was executed by employment of radial arm maze. Finally brain biochemical measures of Acetyl cholinesterase and glutamate along with cresyl violet staining and estimation of total neuronal number of the hippocampus were performed. Both at normal and reduced doses of VAL exhibited augmentation of MI on temporal lobe epileptic mice. Although reduction in VAL dose brought...
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Posted by admin on Sep 30, 2017 in |
Clerodendrum phlomidis is a shrub common in India and Sri Lanka. It is one of the highly traded medicinal plants from tropical forests, as the leaves and roots are used in folk-lore, Ayurveda, Siddha and Unnani medicines. In this study preliminary phytochemical screening, cytotoxic, antioxidant and GC – MS study of leaf of C. phlomidis was evaluated. Successive extraction of the leaf of C. phlomidis was carried out with 5 solvents petroleum ether, chloroform, ethyl acetate, methanol and water. Antioxidant activity was evaluated using DPPH free radical scavenging assay at four different doses of 31.25, 125, 500 and 1000 µg/mL, cytotoxic potential was studied by brine shrimp lethality assay at four different doses of 1, 10, 100 and 1000 µg/mL. GC-MS analysis of methanol leaf extract was carried out on Thermo GC-TRACE ultra version: 5.0, Thermo MS DSQ II. Phytochemical screening analysis of the extracts indicated the presence of secondary metabolites such as steroids, steroidal glycosides, phenols, saponins, coumarins, alkaloids and flavanoids. Methanol leaf extract showed significant cytotoxic potential...
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Posted by admin on Sep 30, 2017 in |
Medicinal properties in Lawsonia inermis are due to the presence of bioactive compounds and can also show adverse effects at high concentrations. In the present study we have determined the nontoxic concentration of hexane, ethyl acetate and methanol fractions of plant leaves. 50 wistar albino rats of both sexes were used as experimental animal and were randomly divided into 4 groups viz. Group 1 (control), Group 2 (Hexane fraction), Group 3 (Ethyl acetate Fraction) and Group 4 (Methanol Fraction). Groups 2, 3 and 4 were further divided into following sub groups, Group 2A (H) (100mg/kg), Group 2B (H) (250mg/kg), Group 2C (H) (500mg/kg), Group 3A (H) (100mg/kg), Group 3B (H) (250mg/kg), Group 3C (H) (500mg/kg), Group 4A (H) (100mg/kg), Group 4B (H) (250mg/kg) and Group 4C (H) (500mg/kg). Rats in Group 1 (control) received 3% DMSO as solvent for dissolving fractions. All the doses were administered orally with the help of polythene cannula and were given once in a day, followed up to 21 days. After 21 days, blood...
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Posted by admin on Sep 2, 2017 in |
A series of new Mannich base derivatives were designed, synthesized by conventional and non-conventional microwave method, compared and evaluated for their antimicrobial and anti-mycobacterial activity. Structures of the newly synthesized compounds were assigned on the basis of elemental analysis, IR, 1H NMR, 13C NMR and mass spectral studies. The bioactive assay showed that Mannich base derivative 1c displayed encouraging anti-tubercular activity in vitro against Mycobacterium tuberculosis H37Ra using MABA method in primary screening. The antibacterial and antifungal efficacy of these derivatives using broth micro dilution method, showed potency for 1c against Escherichia coli, 1g against Staphylococcus aureus, Candida albicans and 1j against Candida albicans in comparison with reference drugs. Furthermore, docking study has been performed for three different PDBs (3JZF, 4NZ9 and 2WHF) of different strains that showed good binding interactions. Moreover, the synthesized compounds were also analyzed for ADME properties and showed potential to build up as good oral drug...
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Posted by admin on Aug 31, 2017 in |
Tuberculosis is caused by Mycobacterium tuberculosis and affects primarily the lungs. Breast tissue is remarkably resistant to tuberculosis. This is due to fact that it gives infertile environment for survival and multiplication of tubercle bacilli like skeletal muscle and spleen. The breast may become infected by variety of ways e.g. haematogenous, lymphatic, spread from contiguous structures, direct inoculation and ductal infection. The path of spread of disease from lungs to breast tissue was traced via trachea-bronchial, Para tracheal, mediastinal lymph trunk and internal mammary nodes. Scrofulous swelling of the bosom is the description given by Sir Astley Cooper who recorded first ever Breast Tuberculosis. It is usually seen in lactating young multiparous women and presents as lump in the central or upper outer quadrant of breast. It is occasionally mistaken as Carcinoma Breast or Breast Abscess. The overall incidence of tuberculosis Mastitis is reported to be 0.1 % of all breast lesions while in developing countries it constituted approximately 3.0 % of surgically treated breast...
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