Posted by admin on Jun 30, 2017 in |
The novel benzimidazole derivatives were synthesized by 2-acetyl benzimidazole consent to react with various substituted aromatic aldehyde (2a–e) to obtain preferred intermediate chalcones (3a–j), these intermediates are undergo the cyclocondensation with guanidine hydrochloride and phenyl hydrazine yielded benzimidazole derivatives (4a–j and 5a–j respectively). The synthesized compounds were analyzed spectral studies to confirm their structure and in-vitro anticancer activity was screened in MCF-7 and CaCo-2. Among them the compounds containing benzimidazole with pyrimidine moiety (4a, 4b, 4d, 4g, 4i) and compounds containing benzimidazole with pyrazole moiety (5b, 5d, 5g, 5i) showed significant activity against MCF-7 and CaCO-2 cell line. In particular compound 4a exhibited IC50 8.22μM in MCF-7 , IC50 5.67μM CaCO-2 cell line. The compound 4a induced apoptosis in Caco-2 cells as evaluated by EB/AO staining and 24 hours Caspase study revealed that compound 4a shows 2 fold activity in caspase 3 and 9 pathway, single fold activity in caspase 8 pathway, which can be regarded as promising anticancer...
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Posted by admin on Jun 30, 2017 in |
Introduction: The health and wellness sector is bracing for a radical change with Information and Communication Technology taking the leading role in research and development, diagnostics, and monitoring. The consumer’s focus is shifting from treatment-based remedies to preventive remedies gradually. Aim: To explore the slow transition from curative to preventive healthcare in the prescribing behavior of physicians and the different perceptions among physicians and patients regarding Nutraceuticals. Methods: A cross-sectional study based on observation was conducted in South Delhi in India. Random sampling was used to divide the physician population into two strata namely physicians practicing in the hospitals and physicians practicing privately. The respondents were interviewed separately and the prescriptions and bills were scrutinized. Pediatricianswere excluded from the study. The data was fed into EXCEL sheet and analyzed based on percentages and proportions. Results: It was found that vitamins comprised the largest chunk with 49% prescribed in the hospitals whereas 40% prescribed in the private clinics. It is found that 40% of the physicians agreed that non-availability was...
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Posted by admin on Jun 30, 2017 in |
The leaves of Corchorus trilocularis possesses several bioactivities and is used in traditional medicinal systems. However, its anti-diabetic activity has not been scientifically investigated so far. The present study was carried out to evaluate the anti-diabetic activity of chloroform extract extract of Corchorus trilocularis (family: Tiliaceae) leaves in Streptozotocin induced diabetic rats. Chloroform extract showed presence of saponins, phytosterols, flavonoids, phenols, steroids and terpenoids. From the toxicity study it was observed that chloroform extract of Corchorus trilocularis (CECT) was nontoxic up to the dose of 2000 mg/kg body weight. In this study, animals received continuous oral administration of CECT for a period of 21 days at the doses of 200 mg/kg and 400 mg/kg body weight. The effect of CECT was compared with oral dose of 5mg/kg Glibenclamide. The results showed that the CECT significantly lowered the blood sugar level of hyperglycemic rats in a dose dependent manner. CECT reduced glycosylated hemoglobin, lactate dehydrogenase and creatinine kinase levels in streptozotocin treated animals. The extract also ameliorated oxidative stress Parameters...
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Posted by admin on Jun 30, 2017 in |
Background: Nagabhasma is a lead-based Ayurvedic medicine. Numerous reports indicate the toxic effects of lead used in this preparations on various systems of our body. But Ayurveda claims that the toxic effect of lead gets nullified thereby acquiring the medicinal property by following stringent and traditional method of preparation. Objectives: Therefore, the present study is designed to evaluate the effect of such detoxified lead in various stages of authentically prepared Nagabhasma on the histopathology of kidney in comparison with lead acetate treated and commercially available Nagabhasma treated animals. Materials and method: Less than the human equivalent doses of Nagabhasma at four different stages of its preparation were fed orally for 30 days and 60 days (short term and long term) to Wistar rats. The animals were even kept under observation for a period of two months after the treatment period and then the residual effect was studied. After the treatment and observation period, the animals were sacrificed to collect the kidneys for histopathological examination. Results: The histopathological results of...
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Posted by admin on Jun 30, 2017 in |
Mycobacterium tuberculosis (Mtb) is a pathogenic bacteria species in the genus Mycobacterium and the causative agent of most cases of tuberculosis. Tuberculosis (TB) is the leading cause of death in the world from a bacterial infectious disease. This antibiotic resistance strain lead to development of the new antibiotics or drug molecules which can kill or suppress the growth of Mycobacterium tuberculosis. The aim of this study is discovery a new drug molecules against the product of accD3 gene which play a vital role in mycolic acid biosynthesis pathway (MAP). As a first step accD3 gene amplified from the complete genome of Mycobacterium tuberculosis Iraqi isolates and sequenced, eight out of twenty of the resulted sequences were chosen to achieve the in silico studies, these sequences have deposited in GenBank database with strain names (ALQM1, ALQM2, ALQM3, ALQM4, ALQM5, ALQM6, ALQM7, ALQM8 ) and Accession numbers (LC006979, LC008196, LC009412, LC009414, LC034168, LC038020, LC041163, LC041368) respectively, two drug molecules resulted from in silico studies and used in the practical application to ...
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