Posted by admin on Apr 30, 2017 in |
A New method was established for simultaneous estimation of Metformin and Empagliflozin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Metformin and Empagliflozin by using Symmetry C18 column (4.6×150mm) 5µ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol: phosphate buffer (KH2PO4 and K2HPO4) phosphate pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength used was Waters HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.403 mins and 3.907 mins. The % purity of Metformin and Empagliflozin was found to be 99.87% and 100.27% respectively. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1). The linearity study of Metformin and Empagliflozin was found in concentration range of 50µg-250µg and 5µg-25µg and correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 99.56% and 99.48%, %RSD for repeatability was 0.3and 0.3, % RSD for intermediate precision was 1.3 and 0.4respectively. LOD value was 2.17...
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Posted by admin on Apr 30, 2017 in |
Pinus roxburghii (F-Pinaceae) is one of the most common chir-pine found in the mid-Himalayan region. The crown of this species displays two different needle colour morphotypes (Dark- Green, Light- Green). It is a well known timber and resin yielding tree. Wood exudes oleoresin which yields rosin and turpentine oil having important role in commercial industry. So, the present study was focused on the identification of superior phenotypes possessing higher quality resin yield from two morphotypes of P. roxburghii of Himachal Pradesh. Trees were selected from different areas of Himachal Pradesh with varying latitude and altitude and were tapped for one month. Needle from the same trees were collected, washed and used for section cutting to determine resin ducts. The needle colour, altitude and latitude had significant influence on these parameter. Results showed that the Dharamshala latitudinal cline -32o to 33o N (L3) has recorded maximum resin yield (544.439g) whereas Kullu – Mandi latitudinal cline -31o to 32o N (L2) observed minimum resin yield (312.795g) and the highly significant resin...
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Posted by admin on Apr 30, 2017 in |
Acinetobacter baumanniiis typically coccobacillus Gram-Negative bacterium, resists almost all antibiotics in use. Synthetic antimicrobial peptides (AMPs) against this bacterium were designed with the aid of computational software and databases to be with the accepted models. Antimicrobial peptides (AMPs) might be the candidate solution for the this problems. Four peptides designated MY1, MY2, MY3 and MY4 were synthesized with length 11-14 amino acids, they were rich in Arginine and Tryptophan. The MICs of the peptides against colistin sensitive A. baumannii were 2.4, 6.2, 6.9, 6.6 µg/ml (mean for 10 strains) respectively vs Mastoparan as control was 10.4 µg/ml, whereas the MICs of peptides against colistin resistant A. baumannii were 3.8, 6.2, 6.8, 6.4 µg/ml (mean for 10 strains) respectively vs Mastoparan 13.6 µg/ml. The designed peptides showed no hemolytic activity at effective MICs. The practical calculated Therapeutic Index were 141.7, 67.7, 60.9, 77.3 vs 7.7 for Mastoparan (Colistin sensitive strains) and for resistant strains were 89.5, 67.7, 61.8, 79.7 vs Mastoparan...
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Posted by admin on Apr 30, 2017 in |
The number of medical applications for natural or synthetic biomaterials contin\ues to expand. One particularly interesting development concerns the use of injectable polymeric biomaterials. The forms in which the biomaterials are injected can vary from particulate matter, e.g., microspheres or irregularly shaped particles, to gels and cements that are injected in liquid form and which harden inside the body. Polymers have played an integral role in the advancement of drug delivery technology by providing controlled release of therapeutic agents in constant doses over long periods, cyclic dosage, and tunable release of both hydrophilic and hydrophobic drugs. The development of using polymer combination have attracted much attention because the biodegradation rate of the copolymer is easily controlled by altering its composition. In this review, the fundamental drug delivery systems from polymer blends including the origins and applications of polymer blend systems and polymer blend therapeutics are highlighted. The PCL microparticle-dispersed PLGA solution may be a good candidate as an injectable bulking agent. Using PCL–PLGA blend, the porous structure of the...
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Posted by admin on Apr 30, 2017 in |
Pterocarpus marsupium Roxb. (Fabaceae) is a moderate to large deciduous tree, belonging to family Fabaceae. It is considered as an extremely useful source of unique natural products for development of medicines against various diseases and for industrial product development. The present study includes physicochemical parameters like ash value, extractive value, fluorescence analysis, moisture content and preliminary phytochemical screening along with mineral analysis. Antimicrobial activity was also assessed for this plant. The physicochemical constants obtained were within normal levels prescribed by standards. The phytochemical studies revealed the presence of primary and secondary metabolites in various solvent extracts of Pterocarpus marsupium bark. The estimated mineral composition was in good amounts which would serve as a device for deciding dosage of ayurvedic drug prepared from the plant. Antimicrobial activity by disc diffusion method indicated the zone of inhibition which ranges from 11-22mm for different extracts. Further evaluation of test samples for minimum inhibitory concentration (MIC) using micro dilution method showed that the methanol extract had exhibited significant activity by inhibiting S.typhi, E.faecalis...
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