A COMPARATIVE INVESTIGATION OF METHANOL AND SILVER NANOPARTICLES EXTRACTION FROM CORIANDRUM SATIVUM SEEDS FOR ANTIDIABETIC POTENTIALITY – IN-VITRO

Botanical name of coriander is Coriandrum sativum Linn. Coriandrum sativum belongs to the family Umbelliferae which is a highly reputed ayurvedic medicinal tree commonly known as Dhania. Essential oils, flavonoids, fatty acids, and sterols have been isolated from different parts of C. sativum so far. The phytochemical analysis of both methanol and AgNP’s extract was evaluated. Results revealed the presence of alkaloids, flavonoids, diterpenes, phenol, and phytosterol in the methanol extract, and AgNP’s extract contained flavonoids, phenols, and phytosterols. The antioxidant activity of both the extract was measured by the ability to scavenging DPPH, phenols, flavonoids, metal chelating, FRAP and was compared with suitable standards. These assays showed that both the extracts exhibit significant antioxidant activity. The highest percentage of inhibition was given by methanol extract when compared to standards, while in DPPH assay, AgNP’s showed the highest inhibition compared to methanol extract. The antibacterial activity of different concentrations of both extracts of C. sativum was determined against different bacterial strains. The methanol and AgNP’s extraction showed maximum inhibition zone against all the tested bacterial strains at higher concentrations. AgNP’s showed the highest inhibition zone compared to methanol extract.  The anti-inflammatory activity of both extracts of C. sativum was evaluated using suitable standards. The methanol extract showed the highest activity than AgNP’s. Alpha-amylase activity did not show any significant inhibition activity with both the extracts. The constituent components were separated in HPLC using a solvent system developed with the aid of TLC. The results of the study support the partial ethano-medical use of C. sativum as antibacterial agent.