A COMPARATIVE STUDY ON THE ANTI-CANCER POTENTIAL OF ENICOSTEMA AXILLARE ON CANINE AND HUMAN BREAST CANCER BY TARGETING RUNX AS A PROGNOSTIC MARKERAbstract
The main objective of this study is to find a cure for Human Breast Cancer having Canine Mammary Tumor as a model using the ethyl acetate extract of Enicostema axillare targeting Runt-Related Transcription factor, which plays a vital role in Hippo Signaling Pathway. The preliminary phytochemical analysis and GC-MS analysis were used to determine the plant extract’s bioactive compounds. The in-vitro Antioxidant and Anti-Proliferation assay was performed on MCF-7 and REM-134 cell lines by MTT assay. The Pharmacokinetics and Pharmacodynamics of Bioactive compounds were studied using Pre-ADMET and PASS SERVER software, respectively. Minimum Antioxidant potential Inhibition of extract was observed at 40µg/mL, and IC50 value calculated by MTT assay was found to be 31.2µg/mL for MCF-7 and 125µg/mL for REM-134. Bioactive compounds obtained from the GC-MS study were subjected to molecular docking on RUNX of Humans and Canine. The Dock score was found to be 96.7 for Humans and 102.13 for Canine. RUNX can be used as a prognostic marker for the treatment of mammary tumors for both Human and Dogs.
S. Pavithra and A. Praveena *
Department of Biotechnology, Prathyusha Engineering College, Tiruvallur, Tamil Nadu, India.
15 August 2022
13 October 2022
31 October 2022
01 April 2023