A MODIFIED EFFICIENT METHOD FOR ISOLATION OF RHEIN FROM CASSIA ANGUSTIFOLIA (SENNA)Abstract
Chemically, rhein is 9, 10-Dihydro-4, 5-dihydroxy-9, 10-dioxo-2-anthracenecarboxylic acid, and use as an intermediate to synthesize diacerein which widely used in the treatment of degenerative diseases like, osteoarthritis, osteoporosis, and rheumatoid arthritis and hence has commercial importance. Rhein occurs in free state in nature in different plant families like Cassia, Rhamnus, and Rheum but in very less concentration and as it is currently subject of interest because of its anti-tumor, anti-viral and anti-oxidant properties, therefore, there is need for simple and efficient isolation and extraction method to obtain rhein from available indigenous plant sources. A modified method developed for the isolation of rhein from Cassia angustifolia leaves is described, the method involves oxidative hydrolysis of sennoside with help of an oxidizing agent followed by the isolation of the rhein from reaction mixture, and with substantial yield improvement is achieved. The objective of this study to optimize yield of the rhein from senna semisynthetically. The isolated rhein was analyzed by spectral analysis for its confirmation.
S. A. Gangurde *, K. S. Laddha and S. V. Joshi
Department of Pharmaceutical Science and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
07 May 2019
04 September 2019
13 November 2019
01 March 2020