A NEW STEBILITY INDICATING RP-HPLC-PDA METHOD FOR SIMULTANEOUS ESTIMATION OF NEOMYCIN AND FLUOCINOLONE IN PHARMACEUTICAL TABLET DOSAGE FORMAbstract
A simple, selective, ingenuous, reasonable, speedy, Reverse Phase High Performance Liquid Chromatography (RP-HPLC) was developed for simultaneous estimation of neomycin and fluocinolone in its tablet dosage form. The separation was eluted using a mobile phase of buffer and methanol in the proportion of 50:50 a pumped at a flow rate of 1 ml/min besides 250 nm as a UV detection wavelength. The stationary phase used was column discovery 250 × 4.6 mm, 5m. The drug samples were eluted at a retention time of neomycin 2.403 min and fluocinolone 3.303 min. The proposed analysis was developed and validated based on ICH guidelines by taking into account the parameters such as precision, accuracy, linearity, specificity, robustness, limit of detection, limit of quantification and degradation studies. The developed RP-HPLC method proved to be stability indicating by the resolution of samples with their forced degradation studies. The designed method used for routine analysis of neomycin and fluocinolone in combined dosage form. For peak detection and purity confirmation PDA was used as a tool.
B. Balaswami *, V. R. Peddakotla, S. R. Bandi and S. Pagidirai
Department of Chemistry, Sri Krishnadevaraya University, Ananthapuramu, Andhra Pradesh, India.
11 February, 2018
17 April, 2018
09 June, 2018
01 October, 2018