A NOVEL METHOD AND VALIDATION FOR THE QUANTIFICATION OF SACCHARINE AND ASPARTAME IN THE LOCAL MARKETED PRODUCTS

Artificial sweeteners are non-nutritive sugar substitutes designed to provide sweetness without the calorie content of sugar. Examples like Saccharin, Aspartame, Neotame Acesulfame K and they are used in producing a low- or no-calorie diet to lose weight. In the last few years, they have be used in most of the products. Now a days artificial sweeteners are used because they have no calories and pose no risk of obesity. It provides the detailed description of some of the artificial sweeteners and their history, uses and adverse effects. They are often used in food and beverages as alternatives to sugar, providing individuals pursuing to reduce their sugar intake. The artificial sweeteners have an important impact on people who have diabetes or overweight. Validation was performed to know the Linearity, Precision, Ruggedness, Robustness, Limit of detection, Limit of quantification. A simple method is used to determine the artificial sweeteners like saccharin and Aspartame based on UV visible spectrophotometric method and this article provides the analysis of Saccharin and Aspartame and including their validation parameters. For Saccharin a simple method developed by using simple distilled water and the linear responses were recorded in the concentration rangesof10µg/ml-90µg/ml with a good correlation coefficient (r²) of 0.9922, and for Aspartame a chromogenic method has been developed that is by using Ninhydrin Reagent, according to the ICH Q2(R2) guidelines and the linear responses were recorded in the concentration ranges of 10µg/ml-175µg/ml with a good correlation coefficient (r²) of 0.9971, the precision was found to be within the acceptable limits (%RSD ≤ 2) for all the methods.