A NOVEL METHOD FOR ESTIMATION OF DIPEPTIDYL PEPTIDASE-4 INHIBITOR IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY VISIBLE SPECTROPHOTOMETRYAbstract
A new, simple and low cost spectrophotometric method for the determination of teneligliptin, a new DPP IV inhibitor drug in bulk and pharmaceutical dosage form using sodium nitroprusside was developed. The absorbance maximum (λmax) of the resulted colored product was at 445 nm. As different solvents were tried methanol was found to be the most suitable solvent. The Beer’s law has been obeyed in the concentration range of 4 – 20 µg/ml with the detection limits of 0.09µg/ml and limit of Quantification of 0.28µg/ml. The correlation coefficient was found to be (r = 0.9999). The reaction ratio between teneligliptin and sodium nitroprusside was studied and found to be 1:1. The method was validated and results obtained for the assay of two different brands of teneligliptin tablets were compared. The repeatability and reproducibility of the developed method were evaluated and the obtained results quoted.
V. Kommineni *, N. P. Lakshmi, B. Ramya and C. B. Sekharan and O. Ahmed
Department of Pharmaceutical Analysis, Sri Venkateswara College of Pharmacy, Hyderabad, Telangana, India.
07 September, 2017
20 November, 2017
21 November, 2017
01 June, 2018