A NOVEL SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF MEMANTINE HYDROCLORIDE IN BULK AND PHARMACEUTICAL FORMULATIONAbstract
The author selected and developed a new simple, sensitive and reproducible spectrofluorimetric method and validated for the determination of Memantine Hydrochloride in bulk as well as pharmaceutical formulations depending on condensation of Memantine hydrochloride with 1, 2 – Naphthoquinone -4- sulphonate (NQS) in an alkaline medium to become a product with orange color. The spectrofluorimetric method involved reduction of the product with potassium borohydrate and the later measurements of fluorescent formed lessen the NQS product after excitation at 295 nm and emission wavelength at 385 nm for the spectrofluorimetric method under optimum conditions, linear relationship with best correlation coefficient 0.9999 and the linearity was detected in between the range of 0.25-1.25 µg/mL. The mean accuracy was found to be 99.57 % to 99.66 %. The intraday and interday precision was found to be 0.14-0.16 % and 0.13-0.15% respectively. The limit of detection was found to be 0.0090µg/mL and the limit of quantification was 0.0270µg/mL. Therefore this newly recommended spectrofluorimetric method is most suitable for estimation of Memantine and observed to be validated for calculation of accuracy, precision, robustness, LOD and LOQ. So this method becomes highly congenial for analyzation of Memantine in tablet form either commercially or in dosage form with reagent of NQS. The results of analysis of this new proposed method can be compared and found to be agreeable with the results achieved through official method. Therefore spectrofluorimetric method is considered as most convenient analytical technical method for pharmaceutical estimation in quality control as well as clinical laboratories
P. Ravisankar *, G. Devala Rao and Ch. Devadasu
Associate Professor Department of Pharmaceutical Analysis and Quality Assurance, Vignan Pharmacy College, Vadlamudi-522 213, A.P., India.
18 April, 2014
04 July, 2014
30 July, 2014
01 November, 2014