A NOVEL STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF N-ACETYLCYSTEINE AND AMBROXOL IN COMBINED TABLET DOSAGE FORMAbstract
A new, simple, sensitive, precise, accurate and stability indicating RP-HPLC method has been developed for the simultaneous determination of N-Acetyl-L-cysteine and Ambroxol Hydrochloride in combined tablet formulation. The combination is mainly used as mucolytic. The study aimed at developing and validating a method which can be used easily and effectively for determination of given drugs in various formulations. The analysis was carried out at 220 nm and the range was obtained as 3-18 µg/ml for AMB and 20-120 µg/ml for NAC. Recovery studies indicated that the drug recovery from the formulation was within acceptable limits. The developed method was statistically evaluated. The %RSD values reported were less than 2 all through the validation which indicates variations in the results are minimal. Thus the proposed method of analysis was found to be simple, sensitive, accurate, precise, rapid and also economical and can be applied successfully for simultaneous estimation of both N-Acetyl-L-cysteine and Ambroxol Hydrochloride in bulk and combined tablet formulation.
V. Kinjawadekar *, S. Boddu and O. Ghadge
Oriental College of Pharmacy, Sanpada, Navi Mumbai, Maharashtra, India.
20 October, 2016
14 December, 2016
16 December, 2016
01 May, 2017