A NOVEL TANNIC ACID FROM GANODERMA LUCIDUM FRUITING BODIES EXTRACT AMELIORATES EARLY DIABETIC NEPHROPATHY IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Aim: The present study evaluated the anti-diabetic, anti-hyperlipidimeic, antioxidant activities and renal protective effect of aqueous extract from Ganoderma. lucidum fruiting bodies in streptozotocin induced diabetic rats. Material and Results: Qualitative and quantitative phytochemical analysis for G. Lucidum aqueous extract showed the Presence of high concentration of tannins content. Thin layer chromatography (TLC) revealed the presence one tannin spot which high performance liquid chromatography (HPLC) analysis confirmed that the tannin compound is tannic acid. Extracted tannic acid showed highly antioxidant activity in vitro. Two doses of the extract were administered intraperitoneally. Tannic acid significantly decrease blood glucose levels and significant increase in serum insulin in addition, it improved serum lipid profile in diabetic rats compared to untreated diabetic group. Results: Showed that tannic acid also had potent antioxidant activity in vivo, as it significantly decreased MDA while GSH and GPx in erythrocytic and renal tissues were significantly increased. Histopathological examination of kidney tissue tannic acid treated rats showed mild glomerular atrophy and minimal tubular affection with noticeably dilated blood vessels compared to untreated diabetic rats. Conclusion: These results suggest that extracted tannic acid has antihyperglycemic, hypolipidemic and renal protective effect against STZ-induced diabetic nephropathy in rats.