A NOVEL USE OF OXIDATIVE COUPLING REACTIONS FOR DETERMINATION OF ANTIBIOTIC AND ANTI-INFLAMMATORY DRUGS IN PHARMACEUTICAL FORMULATIONSAbstract
The present work describes new spectrophotometeric methods for the assay of four drugs namely Meropenem (MPN), Cefditoren Pivoxil (CTP), Mesalamine (MSL) and Cefdinir (CDN) in both pure form and Pharmaceutical formulations. All methods involve the oxidative coupling reaction of MPN, CTP, MSL and CDN with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in presence of Fe(III) in an acidic medium to form colored products with absorption maxima at 620, 630, 500 and 620 nm respectively. Beer’s law was obeyed in the ranges of 5-25, 5-40, 5-25 and 2-12 µg/ml for MPN-MBTH, CTP-MBTH, MSL-MBTH and CDN-MBTH respectively. Statistical treatment of the experimental results indicates that the methods are precise and accurate. The proposed methods have been applied to the determination of the active components in commercial formulations with no interference from excipients. A comparative study between the suggested procedures and reference procedure for these compounds in the commercial formulations showed no significant difference between two procedures.
Srinivasa Rao Narala* and K. Saraswathi2
Department of Chemistry, R.V.R. & J.C. College of Engineering, Guntur, Andhra Pradesh, India
15 September, 2010
02 November, 2010
15, January 2011
01 February, 2011