A SIMPLE AND SENSITIVE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TRACE LEVEL MONO ISOPROPYLAMINE IN PHARMACEUTICAL DRUG SUBSTANCESAbstract
A simple and sensitive spectrophotometric method was developed and optimized for the determination of content of mono isopropylamine at very low levels in Carisoprodol and Isoproterenol hydrochloride. The method was based upon the observation, that characterstic colour results up on addition of colour reagent (consists of Acetyl acetone, formaldehyde and pyridine) and heat for 10 minutes on water bath to form coloured complex. This coloured complex is slightly yellow and its absorption maximum at 420 nm. The optimized method was validated to prove its performance characterstic by demonstrating selectivity, sensitivity(limit of detection and quantification), linearity, precision and accuracy. The established limit of detection and limit of quantification of mono isopropylamine was found to be 3.3µg/ml and 10µg/ml respectively. The present work was aimed to develop a visible spectrophotometric method, which is simple, sensitive, accurate and cost effective to evaluate the quality of the bulk drugs.
C. Nagaraju *, R. Gorla, U. K. Ray, N. Kanteti, Hemant K. Sharma and S. Vidavalur
APL Research Centre-II(A Division of Aurobindo Pharma Ltd), Indrakaran(V), Sangareddy (M), Medak Dist., Hyderabad, Telangana, India
01 August, 2015
16 October, 2015
13 November, 2015
01 February, 2016