A STUDY ON THE ROLE OF DIETARY AGENT MOMORDICA CHARANTIA L ON PROTEIN GLYCATIONAbstract
Protein glycation is a spontaneous post translational modification of proteins by excess sugars causing formation of advanced glycation end products (AGEs) in diabetic individuals and responsible for diabetes complications. A wide variety of anti-glycating agents have been reported & recently there has been interest in natural products with anti-glycation properties. Momordica charantia L (M. Charantia L) has been used historically for medicinal purposes particularly for treatment of diabetes & cancers. M. Charantia L Extract contains potent antioxidant activity and it can be a useful anti-glycating agent. Material & methods: Human serum albumin was used for in vitro glycation. Various concentrations of extract of M. Charantia L were analyzed. Results: Co-incubation of the M. Charantia L. extract with HSA-fructose mixture intensify the fructose mediated glycation of HSA as indicated by increases fluorescence intensity in tryptophan fluorescence &AGE related fluorescence studies. Conclusion: M. Charantia L. seems to aggravate sugar mediated glycation of the protein and need further studies to pinpoint specific bioactive compounds responsible for the observed activities
Gini Garima, Neeraj Kumar Agrawal *, Shagufta Moin and Pankaj Kumar Gupta
Department of Pharmacology, Himalayan Institute of Medical Science, SRH University, Dehradun, Uttarakhand, India.
11 December, 2015
18 January, 2016
13 February, 2016
01 May, 2016