ACTIVE COMPOUND FROM n-HEXANE FRACTION OF RAMPAI (LYCOPERSICON ESCULENTUM) LEAVES ETHANOL EXTRACTAbstract
Lycopersicon esculentum leaves had traditionally been used to stimulate hair growth in Lampung Pesisir tribes, Tanggamus Regency, Lampung, Indonesia. This study aimed to examine the active compounds from the n-hexane L. esculentum leaves. The study based on our previous research showing that the n-hexane had hair growth stimulating effects. The study started with sample collection, extracted by maceration, fractionated using liquid-liquid extraction (LLE) with water, n-hexane and ethyl acetate with a ratio of 1: 1. Isolation of compounds in n-hexane fraction was done by vacuum liquid chromatography (VLC) method using gradient polarity of chloroform: methanol resulting in a subfraction forming a crystal. The crystal was checked by two ways TLC. The obtained pure crystal was then identified with FTIR, LC-MS, and NMR. The identification of the active compound isolates from the n-hexane isolates contained a class of triterpenoid compounds, namely cycloartenol compounds with the molecular formula C30H50O, m/z 425, 3783 with the name IUPAC 9 beta, 19-cyclo-24-lanostane-3beta-ol, (3beta) -9, 19-Cyclolanost-24-en-3-ol. The chemical compound content of n-hexane fraction of L. esculentum leaves with possibly causing anti-alopecia was the cycloartenol compound with the formula of C30H50O. Further verification determination is suggested to compare this compound to the minoxidil compound, either by in-silico or in-vivo analysis.
Z. Oktoba, M. Moektiwardoyo and R. Mustarichie *
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia.
13 January 2019
11 April 2019
19 April 2019
01 May 2019