ALPHA-AMYLASE INHIBITION AND MEMBRANE STABILIZING EFFECT OF THE STEM BARK OF MAESOBOTRYA DUSENII HUTCHINSONAbstract
Introduction: Control of blood glucose level is a critical approach in the management of diabetes mellitus and its complications. This study investigated the α-amylase inhibition and erythrocyte membrane stabilization effect of extracts of Maesobotrya dusenii Hutch. Method: Powdered stem bark of M. dusenii (100g) was extracted with methanol. The plant material (400g) was also successively macerated with N-hexane, ethyl acetate, and methanol. Phytochemical screening was conducted on the powdered stem bark. All the extracts were evaluated for alpha-amylase inhibition at 10, 20, 30, 40 and 50 µg/mL concentrations using acarbose as standard drug and erythrocyte membrane stabilization at 20, 40, 60 and 80 µg/mL concentrations using diclofenac as standard drug. Gas Chromatography-Mass Spectroscopy (GC-MS) of the hexane extract was analyzed. Result: Phytochemical screening showed the presence of carbohydrates, saponins, and triterpenoids. All the extracts showed a dose-dependent response on alpha-amylase inhibition in which crude methanol had a significant activity of 56.7% inhibition. N-Hexane, ethyl acetate, and crude methanol extracts showed erythrocyte membrane stabilizing effect, but crude methanol showed the most activity with 183.3% inhibition of erythrocyte membrane stabilization and IC50 of 70 µg/mL against diclofenac 28.4 µg/mL. The GC-MS revealed hexadecanoic acid and six other compounds that were fully characterized by database. Conclusion: The study showed that crude methanol extract has significant alpha-amylase inhibitory and anti-inflammatory activity than other extracts. The study justifies the use of M. dusenii in the management of diabetes.
S. Mikailu *, M. C. Okorafor and K. A. Abo
Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Nigeria.
28 October 2018
05 February 2019
08 March 2019
01 November 2019