AMNESIC POTENTIALITY OF DIAZEPAM AT LOW DOSE ON ADMINISTRATION VIA TRANSCRANIAL ROUTE IN RODENTSAbstract
The objective of study to evaluate a drug Diazepam applied transcranially to brain targeted drug delivery to screening for its amnesic effects, in different learning and memory paradigms viz., rod walking test, locomotor activity test, elevated plus maze test, water maze test, pole climbing test and pole climbing test on trained animal. Diazepam 4mg/ kg intraperitoneal used as a standard for comparing the test drug diazepam 4mg/ kg applied transcranially, whereas both water for injection intraperitoneally and seasme oil transcranially used as a control group. In rod walking test and locomotor activity test used mice for evaluating transfer latency and mean locomotion for 05 minutes and it was observed that Diazepam transcranially has a significant amnesic effect (p<0.0001) as compared to control, and as same like standard. In elevated plus maze, morris water maze, and the pole climbing test have been used rats for evaluating transfer latency, escape latency and avoidance latency; it was observed that Diazepam transcranial has a significant amnesic effect (p<0.0001) as compared to control, and as same like standard. In conclusion, based on the findings of the present study Diazepam transcranial application is effective in producing amnesia when it evaluated on different learning and memory evaluation model. This can be used as amnesic inducing agent upon transcranial application to the rodent.
Shibnath Kamila*, N. V. Satheesh Madhav and C. N. Sarkar
Bharat Institute of Technology Ibrahimpatnam, Mangalpally, RR. 500010, Telangana, India.
21 May, 2014
21 July, 2014
20 September, 2014
01 January, 2015