AMPLIFICATION AND SEQUENCING OF MITOCHONDRIAL DNA (HVR-I) EXTRACTED FROM 1200 YEARS OLD HUMAN BONE SPECIMENAbstract
Ancient DNA help in better understanding the evolution and migration pattern of humans throughout the world. The present work is a preliminary effort, to extract and amplify the ancient DNA from human bone samples provided by the Anthropological survey of India collected from the Himalayan region in 1952. Many technical difficulties arise during DNA extraction, PCR amplification and sequencing which make it difficult to work and interpret the analyses.Our preliminary result is encouraging, as we have successfully amplified 456 base pair PCR product of mitochondrial DNA hypervariable region I (HVR1) starting from position 15976 to 16431. Our results confirm that the ancient bone specimens harbours M haplogroup signature with considerable similarities to M21ab sub-group specific to East and Southeast Asia. Absence ofnegative amplification confirms that our ancient mitochondrial DNA is contamination free.
Rajeev Kumar Pandey, Deepankar Pratap Singh , Abid Ali 5, G Sudhakar and V.R. Rao*
Professor, Department of Anthropology, University of Delhi, North Campus, Delhi – 110007, India
13 April, 2013
03 June, 2013
14 August, 2013
01 September, 2013