AMYLASE PRODUCTION ON SOLID STATE FERMENTATION BY WILD TYPE AND MUTANT BACILLUS LICHENIFORMIS & ASPERGILLUS NIGER FROM AGRO-WASTESAbstract
Cultures of Aspergillus niger and Bacillus licheniformis were procured from NCL, Pune. Obtained cultures were exposed to UV irradiation. Mutants of Aspergillus niger exposed to 30 and 50 min UV and Bacillus licheniformis exposed to 20 and 30 min UV were selected. Growth of wild type and mutated Aspergillus niger and Bacillus licheniformis was found to be optimum at 48 hrs of incubation time, 30°C of incubation temperature and pH 6. SSF was carried out using two substrates namely banana peel and potato peel. Alpha amylase produced using the two substrates showed good activity but banana peel as a substrate exhibited highest activity of 113.6 (U/ml) in Aspergillus niger mutant exposed to 30 min UV treatment followed by banana peel exhibiting activity of 11.2 (U/ml) in Aspergillus niger mutant exposed to 50 min UV treatment. In this regard, production of amylase which can be used in food & pharmaceutical industry can be increased by using mutants & waste banana peel.
R.N. Shinde*, M.J. Dhangar and R.B. Narwade
K. K. Wagh College of Agricultural Biotechnology Saraswati Nagar, Panchavati, Nashik – 422003, Maharashtra, India
01 February, 2014
29 April, 2014
07 June, 2014
01 July, 2014