AN ECO-FRIENDLY HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ABACAVIR, DOLUTEGRAVIR AND LAMIVUDINE IN ACTIVE PHARMACEUTICAL INGREDIENTS AND MARKETED TABLET FORMULATIONAbstract
Selective and novel method has been optimized to evaluate Abacavir, Dolutegravir and Lamivudinein bulk and formulation by HPLC. The principle analytes were eluted with the conditions of the mobile phase having the Ethanol: Ethyl acetate (80:20, % v/v) using the Lichrosphere RP C8 column (Phenomenex, USA (250 x 4.6 mm, 5μ) analytical column with the 1.0 ml/min flow rate and 10µl sample volume at 260 nm in UV detector. The retention times of Abacavir, Dolutegravir and Lamivudine were 2.31 min, 3.120 min and 4.59min with a total run time of 6 min. The curve indicates correlation coefficient (r2) was superior by having a value nearer to 1.000 with a linear range of 40 µg/ml-130.0 µg/ml for Abacavir, Dolutegravir and Lamivudine. The correlation coefficient (r2) 0.9971for Abacavir, 0.9979 for Dolutegravir and 0.9947 for Lamivudine were found. The LOD and LOQ for the Abacavir, Dolutegravir and Lamivudine were found at 1.40 µg/ml, 3.01µg/ml, 5.84µg/ml, and 4.25 µg/ml, 9.12 µg/ml and 17.71 µg/ml. The developed method was applied for the bulk and formulation.
Gollapalli Nagaraju *, Ritesh Agarwal and Rama Rao Nadendla
Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India.
02 February 2022
09 April 2022
27 April 2022
01 September 2022