AN OVERVIEW ON THIN LAYER CHROMATOGRAPHYAbstract
In the present article attempt has been made to explain the basic ideas and the significance of Thin layer Chromatography (TLC) in different analytical methods. As TLC is less time consuming, low cost, and can be performed with less complicated technique it has a wide application in pharmaceutical analysis. If performed precisely 32 amino acids can be separated by TLC. Also it has a wide application in identifying impurities in a compound. It can be used as a preliminary analytical method prior to HPLC. The concept of TLC is simple and samples usually require only minimal pretreatment. TLC can be used to monitor the progress of a reaction, identify compounds present in a given substance. TLC is also used to separate the identical compounds in a mixture. Many standard methods in industrial chemistry, environmental toxicology, food chemistry, water, inorganic and pesticide analysis, dye purity, cosmetics, plant materials, and herbal analysis rely upon TLC as the preferred approach.
Archana A. Bele* and Anubha Khale
Lecturer, H.K. College of Pharmacy, Near MHADA Complex, Jogeshwari(W), Mumbai, Maharashtra, India
28 July, 2010
16 November, 2010
18 January, 2010