ANALYSIS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF IBUPROFEN TABLETAbstract
A rapid and sensitive method for the determination of the best conditions that allow us to calibrate the ibuprofen in drug is presented. This approach uses high performance liquid chromatography with DAD detector, which is a separation method most used because it is fast, easy, and maintains the nature of the mixture to be separated. The spectra of ibuprofen were recorded using several mobile phases “58%Na2HPO4 (50mmol/l), 42%ACN” and “60%ACN, 40% water “when the pH values are (pH=2.5 and pH=5); the flow rate values are 0.9ml/min -1.5 ml/min with detection wavelength of 240nm; and two stationary phases using the C18 column and Phenyl urea column (25cm x 4.6mm, 0.5µm). The ibuprofen sample: 1 mg of ibuprofen dissolved in 1 mL of water. For this we have tried through our study aiming relying on foreign references.
Zohra Chakrar , Rachid Fegas *, Said Zerkout, Zhor Bettache and Abdalhamid Zerdaoui
Laboratory of Bioactive Products Research and Promotion of Biomass (LPBVB), Ecole Normale Supérieur - KOUBA / Algiers - Algeria
28 October, 2013
09 February, 2014
24 March, 2014
01 April, 2014