ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TRIMETAZIDINE BY EXTRACTIVE SPECTROPHOTOMETRYAbstract
Rapid, simple, sensitive and accurate spectrophotometric methods have been developed for the determination of Trimetazidine in pure and pharmaceutical formulations. Various methods were developed for estimation of trimetazidine .but a simple extractive photometric method using has not been developed .This method is based on the formation of chloroform soluble ion pair complex of Trimetazidine with bromothymol blue (BTB) in buffer of pH 5.0 with absorption maximum at 409nm. Reaction conditions were optimised to obtain the maximum colour intensity. The absorbance was found to increase linearly with increase in concentration of Trimetazidine. Calibration graph was plotted and the correlation coefficient was found to be 0.998.Beer’s law was obeyed in the concentration range of 1- 7 µg/ml with molar absorptivity of 30*10^3 l /mol /cm .Various analytical parameters have been evaluated and the results have been validated by statistical data. This method has been successfully applied for the assay of drug in pharmaceutical formulations. No interference was observed from common pharmaceutical adjuvants.The proposed method is simple, accurate and suitable for quality control applications.
Y. Naga Prasanna*, S. Archana, G.S. Sowjanya, S. Lalitha, M. Lalitha
Research Lab., Aditya Institute of Pharmaceutical Sciences & Research, ADB Road, Surampalem, Kakinada, Andhra Pradesh, India
25 November, 2012
21 January, 2013
23 February, 2013
01 March, 2013