ANTI BACTERIAL ACTIVITY OF THE FLOWERS OF WOODFORDIA FRUTICOSA ON DIFFERENT MICRO-ORGANISMAbstract
Use of plants as a source of traditional healing systems around the world that utilize herbal remedies is an important source for the discovery of new antimicrobials against resistant strains of bacteria. It is being used as a source of medicinal agents for antibacterial, antihelminthic, astringent, emetic, sedative and stimulant. A 10 g dried leaves powder of Woodfordia fruticosa was extracted separately in each different solvent i.e., aqueous, ethanol and methanol. The extract was stored at 4ºC in airtightbottles until further uses. Total 8 strains including gram positive and gram negative bacteria were selected to assess the susceptibility test against the different drug extract. These are Escherichia coli (ATCC-25922), Pseudomonas aeroginosa (ATCC-27853), Salmonella paratyphi (ATCC-9150), Salmonella typhimorium (ATCC-25241), Shigella sonnei (ATCC-25931), klebseilla pneumonia (ATCC-27736), Staphylococcus aureus (ATCC-25923) and Proteus vulgaris (ATCC-6380). The antibacterial activity was performed in vitro using Agar well diffusion assay and diameter of zone of inhibition was measured. The Methanolic shows good zone of inhibition in almost bacteria. The highest zone of inhibition was observed in Methanolic extract in Shigella is 23.0mm, In Ethanolic extract; it was 22.0 mm in Shigella. The results suggest that Ethanolic and Methanolic extracts can be used in the treatment of infection caused by these bacterial strains used in this study.
Satish Kumar*, Asfia Tarannum , Abdul Saleem , Mir. Yousuf Ali and Rajesh
Department of Ilmul Advia, Jamia Tibbia Deoband, Saharanpur, Uttar Pradesh, India
29 March, 2013
02 May, 2013
15 July, 2013
01 August, 2013