ANTI-BACTERIAL EFFICACY OF PHYTO-SYNTHESIZED ZINC OXIDE NANOPARTICLES USING MURRAYA PANICULATA L. LEAF EXTRACTAbstract
Human beings always tried to protect their body from pathogenic microbes, whereas these pathogens always mimic and mutate themselves to infect humans. Nanotechnology is new and upgrading itself as well as enhancing its applicability in pharmaceuticals. Due to their nano-size structure, property drastically changes from their parent elements. Nanoparticles are used widely in biomedical sciences. Focusing on these perspectives, the present study investigates the impact of phyto-synthesized Zinc Oxide Nanoparticles (ZnO NPs) using Murraya paniculata leaf extracts. The characterization of synthesized ZnO NPs was 32 nm with a more optical stable showing peak value at 355 nm (UV-vis). Further, toxicological efficacy of ZnO NPs was checked against human pathogens viz., Salmonella typhimurium (causing typhoid), Staphylococcus aureus (causing tooth decay and rheumatic fever) and Escherichia coli (causing tooth decay in infants and dysentery). The anti-bacterial bioassay was done using a well diffusion method and compared with the standard drug streptomycin. The test pathogens were found more sensitive towards phyto- synthesized ZnO NPs with a Zone of Inhibition Diameter (ZID) value almost more than 22 mm for all three test pathogens as well as compared with earlier reports and found a potential herbal anti-bacterial agent. From the present investigation, the phyto-synthesized ZnO NPs showed promising results and may be used in dentistry as tooth filling agents and as nano-drugs in pharmaceuticals.
Ajay Kumar Tiwari, Saket Jha *, Rahul Agrawal, Sheo Kumar Mishra, Ashok Kumar Pathak, Abhimanyu Kumar Singh, Anupam Dikshit and Ram Rashile Awasthi
Department of Botany, Institute of Professional Studies, University of Allahabad Prayagraj, Uttar Pradesh, India.
12 April 2021
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01 February 2022