ANTI-DIABETIC ACTIVITIES OF ISOLATED COMPOUND BETA-SITOSTEROL FROM THE ETHANOLIC EXTRACT OF STEM OF ANDROGRAPHIS ECHIOIDES

To study the anti-oxidant and anti-diabetic activities of isolated compound beta-sitosterol from the ethanolic extract of stem of Andrographis echioides under the in-vitro model. The isolation was done using column chromatography using gradient elution with different mobile phases. Structural elucidation was carried out on the basis of spectral analysis. Anti-oxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl scavenging assay. The anti-diabetic activity was evaluated by the inhibitory potential of isolated compound beta-sitosterol against alpha-amylase and alpha-glucosidase assays. The study revealed that the beta-sitosterol exhibited significant α-amylase (58.57±0.29) and α-glucosidase (43.85±0.032) inhibitory activities respectively and well compared with standard acarbose drug. The beta-sitosterol showed the best scavenging activity (74.46±0.036) against the tested radicals like 1,1-diphenyl-2-picrylhydrazyl, The infra-red spectrum specific absorption bands for beta-sitosterol viz:  3427.78 cm^‐1 (O‐H stretching.); 2937.40 cm^‐1 (aliphatic C‐Hstretching); 1640.58 cm^‐1 (C=C absorption peak); other absorption peaks includes 1464.31cm^‐1 (CH₂); 1381.56 cm^‐1 (OH def), 1054.11 cm^‐1 (cycloalkane) and 800.97 cm^‐1. Structural elucidation of beta-sitosterol was done by spectrum analysis such as 13C and 1H depth nuclear magnetic resources. Therefore, it is concluded that beta-sitosterol is a potential source for natural anti-oxidant and anti-diabetic compounds and could have potential use in the management of diabetes mellitus.